Mycobiology

  • 제29권4호
  • /
  • Pages.179-182
  • /
  • 2001
  • /
  • 1229-8093(pISSN)
  • /
  • 2092-9323(eISSN)
한국균학회 (The Korean Society of Mycology)
권호 표지

Cloning and Phylogenetic Analysis of Chitin Synthase Genes from Tricholoma matsutake

  • Suh, Seok-Jong (Department of Microbiology, College of Natural Sciences, Kyungpook National University) ;
  • Kim, Il-Hyeon (Department of Microbiology, College of Natural Sciences, Kyungpook National University) ;
  • Nam, Ju-Hyun (Department of Food, Beverage and Culinary Arts, Taegu Technical College) ;
  • Ghim, Sa-Youl (Department of Microbiology, College of Natural Sciences, Kyungpook National University) ;
  • Bae, Kyung-Sook (Korean Collection for Type Cultures, Genectic Resources Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Jong-Guk (Department of Microbiology, College of Natural Sciences, Kyungpook National University)
  • 발행 : 2001.12.31
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초록

Chitin synthases(UDP-N-acetyl-D-glucosamine: chitin 4-$\beta$-N-acetyl-D-glucosaminyl transferase, EC 2.4.1.16) catalyze the synthesis of chitin from UDP-N-acetyl-D-glucosamine. Two zymogenic type of chitin synthase gene(TmCHS1 and TmCHS2) were amplified and its nucleotide sequences were determined. By the amino acid comparison and UPGMA tree grouping, TmChs1 and TmChs2 were classified as class II and class IV chitin synthases respectively. The class II type TmChs1 was grouped with others of Agaricales ectomycorrhizal mushroom. Additionally the phylogenetic tree was well adapted to Hymenomycete previously classified by morphological and physiological characteristics.

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