Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Effect of Metal Ions on the Degradation and Adsorption of Two Cellobiohydrolases on Microcrystalline Cellulose

  • Published : 20010700
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Abstract

To test the metal ion effect, hydrolysis experiments for two cellobiohydrolases (CBHⅠ and CBH Ⅱ) from Trichoderma reesei have been carried out in the presence of 10 mM metal ions, such as Cu++, Mn++, Ca++, Hg++, Ba++, Pb++, and Cd++. The addition of Mn++, Ba++, and Ca++(10 mM) during the hydrolysis of Avicel PH 101 caused an increase in the total reducing sugar (TRS) for CBH Ⅰ by 142, 135, and 114 percent, respectively. Those for CBH Ⅱ increased by 177, 175, and 115 percent, respectively. The Mn++ was the most stimulatory metal ion, whereas Hg++ was the most inhibitory metal ion. The adsorption experiments were performed to investigate how the influence of Mn++ and Hg++ on the hydrolysis is related to the adsorption of cellobiohydrolases on cellulose. The increase in TRS during hydrolysis by adding Mn++ caused an increase in adsorption affinity (Kad) and tightness (ΔHa). While, the decrease of TRS during hydrolysis by adding Hg++ caused a decrease in the adsorption affinity (Kad) and tightness (ΔHa). These results indicate the changes in the tightness and affinity of adsorption by adding metal ions play a crucial role in the degradation of the microcrystalline cellulose.

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