Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Three Binding Sets Analysis of $\alpha$-Lactalbumin by Interaction of Tetradecy Trimethyl Ammonium Bromude

  • M.R.Housainfokht
  • Published : 20010200
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Abstract

The interaction between tetradecyl trimethyl ammonium bromide (TTAB) with bovine ${\alpha}-lactalbumin$ has been investigated at pH = 9 and at $37^{\circ}C$ by isothermal titration calorimetry, equilibrium dialysis and UV-Vis spectrophotometry methods. The binding data from unusual Scatchard plot have been analyzed in terms of the Hill equation for three sets of binding sites. The calorimetric data show that TTAB interacts endothermically with ${\alpha}-lactalbumin$ and causes protein unfolding below 2 mM concentration of TTAB, which is confirmed by spectrophotometric data. The unfolding of the protein would be mainly due to occupation of the second set of binding sites.

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