The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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Protective Mechanism of Hominis Placenta Extract Against ${H_2O_2}-Induced$ Apoptosis in $PGT-{\beta}$ Cells

$PGT-{\beta}$ 세포주에서 자하거 약침액의 $H_2O_2$로 인한 Apoptosis에 대한 방어 기전

  • Jung-Chul, Seo (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dong-Eui University) ;
  • Jae-Dong, Lee (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung Hee University) ;
  • Dong-Suk, Park (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung Hee University) ;
  • Sung-Keel, Kang (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung Hee University) ;
  • Byung-Cheol, Ahn (Dr. Ahn's Clinic of Oriental Medicine) ;
  • Ee-Hwa, Kim (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Se-Myung University) ;
  • Soon-Ae, Kim (Kohwang Medical Research Institute, Kyung Hee University) ;
  • Hee-Jae, LeeK (ohwang Medical Research Institute, Kyung Hee University) ;
  • Chang-Ju, Kim (Kohwang Medical Research Institute, Kyung Hee University)
  • Published : 2001.09.01
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Abstract

목적 :본 연구는 최근 임상에서 많이 사용하는 자하거 약침액이 과산화수소($H_2O_2$)로 야기된 송과선 세포의 Apoptosis에 있어서 세포 보호에 미치는 영향과 그 기전을 분석하였다. 방법 :송과선 세포주에서 자하거 약침액의 $H_2O_2$로 인한 Apoptosis에 대한 방어 기전을 관찰하기 위하여 면역세포화학법. 세포화학법 및 reverse transcription-polymerase chain reaction (RT-PCR)을 시행하였다. 결과 : 자하거 약침액 투여군에서는 nuclear factor kappa B (NFkB), inducible nitric oxide synthase (iNOS), nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase의 발현이 $H_2O_2$ 투여군보다 감소하였다. RT-PCR에서는 caspase-3의 발현이 자하거 약침액 투여군에서 $H_2O_2$ 투여군보다 억제되었다. 결론： 이상의 결과를 통하여 자하거 약침액이 $H_2O_2$로 유발된 Apoptosis에서 세포보호 효과가 있으며 그 기전은 iNOS와 caspase-3의 억제에 기인할 가능성을 시사한다고 하겠다.

Keywords

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