The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
권호 표지

The Effects of Woohwangcheongsim-won on Reperfusion Following Middle Cerebral Artery Occlusion in Rats

우황청심원이 중대뇌동맥 결찰로 유발된 뇌허혈에 미치는 영향

  • 조규선 (동국대학교 한의과대학 내과학교실) ;
  • 정승현 (동국대학교 한의과대학 내과학교실) ;
  • 신길조 (동국대학교 한의과대학 내과학교실) ;
  • 이원철 (동국대학교 한의과대학 내과학교실)
  • Published : 2001.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : The purpose of this investigation is to evaluate the effects of Woohwangcheongsim-won on reperfusion following MCA occlusion in rats. Methods : To evaluate the effect of Woohwangcheongsim-won on reperfusion following MCA occlusion, the volume of cerebral ischemia and edema were measured and the change of the CAI pyramidal neuron in the hippocampus was investigated by light microscopy. And the changes of several neurotransmitters and enzymes were investigated with the immunohistochemical methods. Results : 1. The volume of the control group, which was ischemic-damaged was 23.6%, and that of the sample group was 13.5%. 2. The voluminalratio of the right/left hemisphere was 116 in the control group, and that of the sample group was 107. 3. The pyramidal cells of CAI area in the control group were greatly damaged. The cells were changed into discontinuous and unsystematic forms, and nuclei, and cytoplasms were shrunk. On the other hand, the cells of the sample group were less damaged. 4. On the immunohistochemical methods, the sensitivities of GABA, NOS, DBH in the control group were increased, and those of synapsin and $eEF-l{\alpha}$ were decreased as compared with the normal group. NOS and DBH which were negative in the normal group showed positive reaction. On the other hand, the sensitivities of GABA, NOS and DBH in the sample group were decreased, but those of NPY, synapsin, CaMKII and $eEF-l{\alpha}$ were increased as compared with the control group. Conclusions : Woohwangcheongsim-won reduced the volume of cerebral ischemia and edema, and minimized the damage of pyramidal cells. The mechanism was related to protein synthesis, such as synapsin, ${\alpha}CaMKII$ and $eEF-l{\alpha}$, which resist neurotoxicity of glutamate receptors.

Keywords

References

  1. Brain Res. v.239 no.1 Delayed neuronal death in the gerbil hippocampus following ischemia Kirino, T.
  2. Neuroscience v.68 no.4 Neurochemical changes following occlusion of the MCA in rats Allen, G.B.;Cheung, R.T.;Cechetto, D.F.
  3. 신경외과학 대한신경외과학회
  4. 중풍학술대회논문집 중풍(뇌졸중) 김영석
  5. 太平惠民和劑局方 陳師文
  6. 東醫寶鑑 許浚
  7. 濟衆新編 康命吉
  8. 대한한의학회지 v.15 no.1 의식장애를 동반한 졸중풍 급성기 환자에서 우황청심원 투여에 관한 고찰 김동웅;오승환;안일회;이언정;이형균;이승무;나성수
  9. 우황청심원이 백서의 뇌손상에 미치는 영향 홍석
  10. 약학회지 v.41 no.6 신우황청심원의 뇌허혈 및 중추신경계에 대한 약효 조태순;이선미;이은방;조성익;김용기;신대희;박대규
  11. Stroke v.20 no.1 Reversible middle cerebral artery occlusion without craniectomy in rats Longa, E.Z.;Weinstein, P.R.;Carlson, S.;Cummins, R.
  12. 경희의학 v.19 우황청심원과 소합향원의 효능에 대한 연구 남상경;이경섭
  13. 우황청심원이 흰쥐의 적출심장에 미치는 영향 고창남
  14. 方藥合編解說 신재용
  15. 우황청심원이 평활근에 미치는 영향 고용석
  16. 생약학회지 v.18 no.4 우황청심원의 순환기계에 미치는 영향 홍남두;김남재;김규섭
  17. 우황청심원이 NOS inhibitor에 의한 흰쥐의 학습 및 기억장애에 미치는 영향 백지성
  18. 고혈압에 우황청심원과 Hydralazine, Atenolol, Captopril 및 Furosemide의 병용투여에 관한 실험적 연구 이영빈
  19. 우황청심원이 인체내피세포의 NOS 및 세포 부착인자 유전자 발현에 미치는 영향에 관한 분자 생물학적 연구 문상관
  20. 대한한의학회지 v.11 no.2 우황청심원의 제형별 임상에 관한 연구 이원철;김영석;배형섭;구본홍
  21. TiPS v.17 Emerging treatments of stroke in humans Walter J. Jorosgetz;Nichael A. Moskowitz
  22. Acta Neuropathol v.64 Delayed neuronal death in the rat hippocampus following transient forebrain ischemia Kirino, T.;Tamura, A.;Sano, K.
  23. Masui. v.47 no.6 The pathophysiology of ischemic neuronal injury Iijima, T.
  24. 동국논집 v.15 허혈성 흰쥐 해마에서 NMDA 수용체 아단위 NR2B와 세포골격단백질 MAP-2의 변화 정용욱;문일수;고복현
  25. 의학신경해부학 박경아;이원택
  26. 신경전달물질 서유헌
  27. J Cell Biol. v.96 no.5 Synapsin I(protin I), a nerve terminal-specific phosphoprotein. III. Its association with synaptic vesicles studied in a highly purified synaptic vesicle preparation Huttner, W.B.;Schieble, R. W.;Greengard, P.;De Camilli, P.
  28. Neurochem Res. v.24 no.10 Effects of neonatal hypoxia on the in vitro phosphorylation of synapsin 1 in rat synaptosomes Moretto, M.B.;de Mattos-Duta, A.;Arteni, N.;Meirelles, R.;de Freitas, M.S.;Netto, C.A.;Pessoa-Pureur, R.
  29. Eur J Biochem. v.146 no.1 Regulation of the activity of eukaryotic peptide elongation factor 1 by autocatalytic phosphorylation Tuhackova, Z.;Ullrichova, J.;Hradec, J.
  30. Genes Cells v.4 no.9 Overproduction of elongation factor 1 alpha, an essential translational component, causes aberrant cell morphology by affecting the control of growth polarity in fission yeast Suda, M.;Fukui, M.;Sogabe, Y.;Sato, K.;Morimatsu, A.;Arai, R.;Motegi, F.;Miyakawa, T.;Mabuchi, I.;Hirata, D.
  31. J Neurosci. v.17 no.10 Glutamate-dependent phosphorylation of elongation factor-2 and inhibition of protein synthesis in neurons Martin, P.;Nastiuk, K.L.;Daniel, N.;Girault ,J.A.;Czernik, A.J.;Glowinski, J.;Nairn, A.C.;Premont, J.
  32. Science v.265 Effects of cerebral ischemia in mice deficient in neuronal nitric oxide synthase Huang, Z.;Huang, P.L.;Panahian, N.;Dalkara, T.;Fishman, M.C.;Moskowitz, M.A.
  33. Eur J Pharmaco. v.172 NMDA receptor activation induces nitric oxide synthesis from arginine in rat brain slices Garthwaite, J.;Garthwaite, G.;Palmer, R.M.J.;Moncada, S.
  34. 신경전달물질과 약물 Zygmunt L. Kruk;Christopher J.Pycock
  35. Endocrinology v.137 no.7 Neuropeptide Y Y-2 receptors hypothalamic in neuroendocrine areas are up-regulated by estradiol and decreased by progestrone cotreatment in the ovariectomized rat Parker, S.L.;Carroll, B.L.;Kalra, S.P.;Stpierre, S.;Fournier, A.;Crowley, W.R.
  36. Brain Res Bull v.34 no.6 Time-dependent changes of vasoactive substances in rat cerebral ischemia Mizushima, J.;Sasaki, K.;Shimazu, M.;Arai, Y.;Matsumoto, K.;Shioda, S.;Nakai, Y.
  37. Neuroscience v.68 no.4 Neurochemical changes following occlusion of the middle cerebral artery in rats Allen, G.V.;Cheung, R.T.;Cechetto, D.F.
  38. J Biol Chem. v.260 no.15 Distinct forebrain and cerebellar isozymes of type II $Ca^{2+}$ dependent protein kinase associate differently with the postsynaptic density fraction Miller, S.G.;Kennedy, M.B.
  39. J Cereb Blood Flow Metab. v.15 no.3 Effect fo cerebal ischemia on calcium/calmodulin-dependent protein kinase II activity and phosphorylation Shackelford, D.A.;Yeh, R.Y.;Hsu, M.;Buzsaki, G.;Zivin, J.A.
  40. J Cereb Blood Flow Metab. v.16 no.1 Ischemia-induced neuronal damage:a role for calcium/calmodulin-dependent protein kinase II Waxham, M.N.;Grotta, J.C.;Silva, A.J.;Strong, R.;Aronowski, J.