한국작물학회지 (KOREAN JOURNAL OF CROP SCIENCE)
- 제46권5호
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- Pages.401-406
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- 2001
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- 0252-9777(pISSN)
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- 2287-8432(eISSN)
Non-destructive Method for Selection of Soybean Lines Contained High Protein and Oil by Near Infrared Reflectance Spectroscopy
- Choung, Myoung-Gun (National Yeongnam Agricultural Experiment Station, RDA) ;
- Baek, In-Youl (National Yeongnam Agricultural Experiment Station, RDA) ;
- Kang, Sung-Taeg (National Yeongnam Agricultural Experiment Station, RDA) ;
- Han, Won-Young (National Yeongnam Agricultural Experiment Station, RDA) ;
- Shin, Doo-Chull (National Yeongnam Agricultural Experiment Station, RDA) ;
- Moon, Huhn-Pal (National Yeongnam Agricultural Experiment Station, RDA) ;
- Kang, Kwang-Hee (College of Natural Resources, Yeungnam University)
- 발행 : 2001.12.01
초록
The applicability of non-destructive near infrared reflectance spectroscopic (NIRS) method was tested to determine the protein and oil contents of intact soybean [Glycine max (L.) Merr.] seeds. A total of 198 soybean calibration samples and 101 validation samples were used for NIRS equation development and validation, respectively. In the developed non-destructive NIRS equation for analysis of protein and oil contents, the most accurate equation was obtained at 2, 8, 6, 1(2nd derivative, 8 nm gap, 6 points smoothing, and 1 point second smoothing) and 2, 1, 20, 10 math treatment conditions with Standard Normal Variate and Detrend (SNVD) scatter correction method and entire spectrum (400-2500 nm) by using Modified Partial Least Squares (MPLS) regression, respectively. Validation of these non-destructive NIRS equations showed very low bias (protein: 0.060%, oil: -0.017%) and standard error of prediction (SEP, protein: 0.568 %, oil : 0.451 %) as well as high coefficient of determination (