식물조직배양학회지 (Korean Journal of Plant Tissue Culture)

한국식물생명공학회 (The Korean Society of Plant Biotechnology)
권호 표지

원형질체 배양을 통한 배추 〔Brassica campestris ssp. perkinensis〕캘러스 형성 및 뿌리분화

Callus Formation and Rooting of Inbred Lines of Chinese Cabbage (Brassica campestris ssp. perkinensis) Though Protopalst Culture

  • 염옥희 (강원대학교 식품응용과학부 & 한국감자 육종소재 은행) ;
  • 전익조 (강원대학교 식품응용과학부 & 한국감자 육종소재 은행) ;
  • 김혜진 (강원대학교 식품응용과학부 & 한국감자 육종소재 은행) ;
  • 백남권 (중앙종묘(주) 오산육종연구소) ;
  • 임학태 (강원대학교 식품응용과학부 & 한국감자 육종소재 은행)
  • 발행 : 2001.05.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

기내에서 성장한 배추의 자엽, 하배축과 계대배양한 지 2~3주 되는 어린잎이 원형질체 분리재료로 사용되었다. 배추의 원형질체 분리에 가장 적합한 효소의 조합은 0.4M mannitol 을 삼투조절제로 한 1% Cellulysin과 0.5% Macerozyme의 효소조합이 배추 원형질체 분리에 가장 적합한 것으로 나타났으며, 잎조직을 27$\pm$1$^{\circ}C$에서 30rpm의 속도로 12~16시간 동안 효소와 반응시켰을 때 7.6$\times$$10^{5}$ protoplast/g의 가장 많은 원형질체를 얻었다. 세포분열을 유기시키기 위하여 K8p 배지에 5 mg/L 2,4-D와 2 mg/L에 첨가하였을 때 배양 7~10일에 자엽과 하배축에서 분리한 원형질체에서 세포군들이 형성되었다. 분열한 세포가 8~10 세포크기 단계에 이르면 0.2% agarose 반고체 배지에 고정시켜 배양하였다. 얻어진 calli들을 100가지 다양한 재분화 배지에 옮겼으나 식물체의 재분화는= 이뤄지지 않았지만, 간혹 캘러스에서 뿌리가 분화되는 것은 관찰할 수 있었다.

Protoplasts were isolated from hypocotyls, cotyledons, and young leaves of Chinese cabbage grown under in vitro environmental condition. An enzyme mixture of 1% Cellulysin and 0.5% Macerozyme in combination with 0.4 M mannitol was most effective condition for protoplast isolation. The highest yield of protoplasts, 7.6$\times$10$^{5}$ protoplast/g of fresh weight, was obtained from the treatment of leaves for 12~16 hours at 27~28$^{\circ}C$ with shaking at 30 rpm. The most suitable medium for an initial cell division was K8p basal medium supplemented with 5 mg/L 2,4-D and 2 mg/L kinetin. Within 7~10 days, protoplasts derived from hypocotyl and cotyledon tissues formed cell colonies. When the cells were grown at the size of 8~10 cells, they were embedded into semi-solid medium containing 0.2% agarose. Calli derived from protoplast culture were transferred to the 100 different types of plant regeneration media, but no completely regenerated plants from inbred lines of Chinese cabbage used for this study wore obtained, though frequent rooting took place in several media tested.

키워드

참고문헌

  1. Theor Appl Genet v.100 Somatic cell hybridization of 'oxy' CMS Brassica juncea (AABB) with B. oleracea(CC)for correction of chlorosis Brassicas Arumugam N;Mokhopadhyay A;Gupta V;Sodhi YS;Verma JK;Pental D;Pradhan AK
  2. Theor Appl Genet v.94 Production of new CMS Brassica oleracea by transfer of 'Anand' cytoplasm from B. rata through protoplast fusion Cardi T;Earle ED
  3. Plant protoplasts Protoplast isolation and culture Eriksson TR;Fowke LC(ed.)
  4. High growth and regeneration capacity of hypocotyl protoplasts in the genus Brassica Physiol Plant v.61 High growth and regeneration capacity of hypocotyl protoplasts in the genus Brassica Physiol Plant Glimelius K
  5. Plant Sci v.45 Selection and enrichment of plant protoplast het erokaryons of Brassicaceae by flow sorting Glimelius K;Mats D;Hugo FF
  6. Plant Cell Rep v.14 Novel flowering and fatty acid characters of rapid cycling Brassica napus L. resynthesized by protoplast fusion Hanen LN;Earle ED
  7. Theor Appl Genet v.91 Transfer of resistance of Xanthominas campestris pv campestris into Brassica oleracea L. by protoplast fusion Hansen LN;Earle ED
  8. J. Japan. Soc Hort Sci v.61 no.1 Callus formation and plant regeneration from protoplast derived from cotyledons and hypocotyls of radish (Raphanus sativus L.) and other Cruciferous plants Hegazi H;Hegazi;Matsubara S
  9. Planta v.126 Nutritional requirements for growth of Vicia hajastana cells and protoplast at very low population density in liquid media Kao KN;Michcharyluk MR
  10. Plant Cell Rep v.9 Efficient plant regeneration from hypocityle protopalsts of broccoli (Brassica oleracea L. ssp. Italica Plenck) Kao HM;Keller WA;Gleddie S;Brown GG
  11. Plant Cell, Tissue and Organ culture v.20 Rapid plant regeneration through organogenesis and somatic embrogenesis from cultured protoplasts of Brassica juncea Kirti PB;Chopa VL
  12. Korean Society of Plant tissue culture v.22 Plant regeneration from hypocotyl-derived protoplasts of Brassica oleracea var capitata Lee YH;Cho HS;Suh SC;Kim HI
  13. J. of Plant Biotechnology v.3 no.1 Production and Characterizations of Somatic Hybrids between Brassica campestris L. ssp perkinensis and Brassica oleracea L. var capitata Lian YJ;Lim HT
  14. J. of Plant Biotechnology v.3 no.1 Plant Regeneration of B. juncea Through Plant Tissue and Protoplast Culture Lian YJ;Lim HT
  15. Plant Cell, Tissue and Organ culture v.19 Studies of protoplast culture and plant regeneration from commercial and rapid cycling Brassica species Loudon PT;Nelson RS;Ingram DS
  16. Physiol Plant v.15 A revised medium for rapid grown and bioassays with tobacco cultures Murashige T;Skoog F
  17. Plant cell, Tissue and Organ culture v.32 Rapid and efficient plant regeneration from hypocotyl protoplasts of Brassica nigra Narasimhulu SB;Kriti PB;Prakash S;Chopra VL
  18. Theor Appl Genet v.93 The morphology, cytology, and C-banded karyotypes of Brassica campestris, B. oleracea, B. napus plants regenerated from protoplasts Olin-Fatih
  19. Plant Cell, Tissue and Organ culture v.17 Regeneration of plants from hypocotyl protoplasts of rapeseed (Brassica napus L. var Oleifera) cultivars Poulsen GB;Nielsen SVS
  20. Biotechnology in Agriculture and Forestry v.9 Regeneration plant of Brassica juncea (L.) CzemεCoss (Brown Mustad) Pua EC;Bajaj YPS(ed.)
  21. Theor Appl Genet v.100 Improved resistance to bacterial soft rot by protoplast fusion between Brassica rapa and B. oleracea Ren JP;Dickson MH;Earle ED
  22. Theor Appl Genet v.94 Direct transfer of a cold-tolerant Ogura male-sterile cytoplasm into cabbage (Brassica oleracea ssp. capitata) via protoplast fusion Sigareva MA;Earle ED
  23. Plant Cell Rep v.13 Studies on Plant regeneration from cotyledonary protoplasts in Brassica campestris Zhao KN;Malcolm I;Whitecross;Dennis J;Bittisnich