Amino Acid Sequence Homology of Hybrid Poplar O-methyltransferuse Involved in Lignin Biosynthesis

  • Park, Young-Goo (Department of Forestry, College of Agriculture, Kyungpook National University) ;
  • Sul, Ill-Whan (Department of Forest Resources, Taegu Polytech College) ;
  • Shin, Dong-Ill (Department of Plant Genetic Engineering, Catholic University of Daegu) ;
  • Park, Jang-Won (Department of Natural Resources, Taegu University) ;
  • Park, Hee-Sung (Department of Plant Genetic Engineering, Catholic University of Daegu)
  • 발행 : 2001.09.01

초록

In $\lambda$-Zap II vector system, a cDNA library was constructed for the developing secondary xylem mRNA from hybrid poplar, Populus nigra x maximowiczii. A cDNA clone of 1.5 kb in size, pOMTB1.4 encoding a lignin-bispecific O-methyltransferase was screened by plaque hybridization using a probe of 540 bp cDNA amplified by polymerase chain reaction from the cDNA library and identified by nucleotide sequencing. Its nucleotide sequence contains one open reading frame of 366 amino acids. The deduced amino acid sequence in comparison with that of Populus tremuloides showed the differences of 9 amino acids and revealed 85-99% homology among alfalfa, poplar and aspen.

키워드

참고문헌

  1. Plant Mol Biol v.17 cDNA cloning sequence analysis and seasonal expression of lignin-bis-pecific caffeic acid/5-hydroxyferulic acid O-methyltrans-ferase of aspen Bugos RC;Chiang VL;Campbell WH
  2. Plant Mol Biol v.20 Structure and expression of the lignin O-methyltrans-ferase gene from Zea mays L. Collazo P;Montoliu L;Puigdomenech P;Rigau J
  3. Plant genetic transformation and Gene expression Draper J;Scott R;Armitage P;Walden R
  4. Plant Physiol v.98 Nucleotide sequence of a complementary DNA encoding O-methyltransferase from poplar Dumas B;Van Doorsselaere J;Gielen J;Legrand M;Fritig B;Van Montagu M;Inze D
  5. Plant Physiol v.97 Stress responses in alfalfa (Medicago sativa L.). X. Molecular cloning and expression of S-adenosyl-L-methionine: caffeic acid-O-methyl-transferase, a key enzyme of lignin biosynthesis Gowri G;Bogos RC;Campbell WH;Maxwell CA;Dixon R
  6. The Biochemistry of Plants v.7 Lignins Grisebach H;PK Stumpf(ed.);EE Conn(eds.)
  7. Annu Rev Plant Physiol Plant Mol Biol v.40 Physiology and molecular biology of phenylpropanoid metabolism Hahlbrock K;Scheel D
  8. Biosynthesis and Biodegradation of Wood Components Biosynthesis of lignin Higuchi T(ed.)
  9. Wood Sci Technol v.24 Lignin biochemistry: biosynthesis and biodegradation Higuchi T
  10. Mol Plant Microbe Interact v.4 Regulation of enzymes involved in lignin biosynthesis: Induction of O-methyltransferase mRNAs during the hypersensitive reaction of tobacco to tobacco mosaic virus Jaeck E;Dumas B;Geoffroy P;Favet N;Inze D;Van Montagu M;Fritig B;Legrand M
  11. Gene v.178 Cloning and sequence analysis of laccase-encoding cDNA clones from tobacco Kiefer-Meyer MC;Gomord V;O'Connell A;Halpin C;Faye L
  12. Plant Physiol v.107 Nucleotide sequence of a cDNA clone encoding an acidic laccase from sycamore maple(Acer psedoplatanus L.) LaFayette PR;Eriksson KEL;Dean JFD
  13. Annu Rev Physiol Plant Mol Biol v.41 Lignin: occurrence, biogenesis and biodegradation Lewis NG;Yamamoto E
  14. Korean J Life Science v.8 Seasonal expression of OMT gene in relation to lignin biosynthesis in two poplar species Park YG;Park HS;Choi JW;Sul IW;Chung Ik;Shin DI
  15. Eur J Biochem v.259 Biochemical and characterization, molecular cloning and expression of laccase- a divergent gene family- in poplar Ranocha P;McDougall G;Hawkin S;Sterjiades R;Borderies G;Stewart D;Cabanes-Macheteau M;Boudet AM;Goffner D
  16. Structure and Reactions Lignins in the plant kingdom. Classification and distribution Lignins: Occurrence, Formation Sarkanen KV;Hergert HL;Ludwig S(eds.)