Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지

Transient and Stable Transformation of Rice (Oryza sativa L.) Calli through Tissue Electroporation

  • Muniz de Pdua, Vnia L. (UFRJ, Instituto de iologia, Dept. Genetica, Cidade Universitria, Brazil) ;
  • Mansur, E. (Universidade do Estado do Rio de Janeiro, Instituto de Biologia, LABMIT/DBCG, Brazil)
  • Published : 2001.09.01
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Abstract

Electroporation of microcalli and embryonic axes of a Brazilian Indica rice cultivar was performed. Some parameters influencing the recovery of transformed callus have been defined through transient npt II expression. Such parameters included the presence of light during incubation of microcalli used as target for electroporation, heat shock at 45$^{\circ}C$, macerozyme pre-digestion of target tissues and the number of pulses during electroporation. Transgenic calli were obtained from embryonic axes after electroporation with plasmid pDM302, which encodes the gene phosphinotricin acetyl transferase (bar) under the control of Act-1 promoter. Integration of the introduced gene into the genome was demonstrated by Southern blot hybridization.

Keywords

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