Journal of Plant Biotechnology

한국식물생명공학회 (The Korean Society of Plant Biotechnology)
권호 표지

Plant Regeneration from Embryogenic Suspension Cultures of Soybean (Glycine max L. Merrill)

  • Jang, Gi-Won (Institute of Agricultural Science and Technology, Chonnam National University) ;
  • Park, Ro-Dong (Department of Agricultural Chemistry, Chonnam National University) ;
  • Kim, Kwang-Soo (Department of Horticulture, Chonnam National University)
  • 발행 : 2001.05.01
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초록

In order to establish efficient plant regeneration from embryogenic suspension cultures of soybean, Glycine max L, we examined the effects of auxin type and concentration, cytokinin type and concentration, and amino acid type and concentration on the growth of embryogenic clumps from induced callus, and the effect of desiccation of mature somatic embryos obtained from these clumps on the frequency of somatic embryo germination. Embryogenic callus was induced from the edge of the cotyledons cultured on MS medium containing 6% sucrose, 40 mg/L 2,4-D, 0.2% gelrite and pH 5.7. The growth of embryogenic clumps was best in early staged, embryogenic callus that was placed in suspension culture of MS medium containing 5 mg/L 2,4-D and 0.5 mg/L asparagine. Single somatic embryos were isolated from the clumps and plated on the same medium for maturation. When the mature single somatic embryos were desiccated for 96 h, somatic embryo germination came up to approximately 90%. The plantlets germinated after embryos desiccation for 2 weeks were transfered to MS medium containing 3% sucrose,0.2% gelrite and pH 5.7.

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참고문헌

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