Journal of Plant Biotechnology

한국식물생명공학회 (The Korean Society of Plant Biotechnology)
권호 표지

Establishment of Genetic Transformation System and Introduction of MADS Box Gene in Hot Pepper (Capsicum annuum L.)

  • Lim, Hak-Tae (Division of Applied Plant Sciences & Center for the Korea Potato Genetic Resources(KPGR), Kangwon National University) ;
  • Zhao, Mei-Ai (Division of Applied Plant Sciences & Center for the Korea Potato Genetic Resources(KPGR), Kangwon National University) ;
  • Lian, Yu-Ji (Division of Applied Plant Sciences & Center for the Korea Potato Genetic Resources(KPGR), Kangwon National University) ;
  • Lee, Ji-Young (Division of Applied Plant Sciences & Center for the Korea Potato Genetic Resources(KPGR), Kangwon National University) ;
  • Eung-Jun park (Division of Applied Plant Sciences & Center for the Korea Potato Genetic Resources(KPGR), Kangwon National University) ;
  • Chun, Ik-Jo (Division of Applied Plant Sciences & Center for the Korea Potato Genetic Resources(KPGR), Kangwon National University) ;
  • Yu, Jae-Woong (Dept. of Horticulture, Seoul National University) ;
  • Kim, Byung-Dong (Dept. of Horticulture, Seoul National University)
  • 발행 : 2001.05.01
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초록

In vitro plant regeneration of inbred breeding line of hot pepper (Capsicum annuum L.) was established using leaf and petiole segments as explants. About 28 days old plants were excised and cultured on MS medium supplemented with TDZ and NAA or in combination with Zeatin. In all of the media compositions tested, combination of TDZ 0.5 mg/L, Zeatin 0.5 mg/L, and NAA 0.1 mg/L was found to be the best medium for shoot bud initiation. Young petiole was the most appropriate explant type for the plant regeneration as well as genetic transformation in hot pepper. In this study, HpMADS1 gene isolated from hot pepper was introduced using Agrobacterium-mediated transformation system. Based on the analysis of Southern blot and RT-PCR, HpMADS1 gene was integrated in the hot pepper genome. It has been known that floral organ development is controlled by a group of regulatory factors containing the MADS domain. Morphological characteristics in these transgenic plants, especially flowering habit, however, were not significantly altered, indicating this MADS gene, HpMADS1 may be non-functional in this case.

키워드

참고문헌

  1. Plant Cell Reports v.10 In vitro plant regeneration from cotyledon and hypocotyl segments in two pepper cultivars Arroyok R;Revilla R
  2. Plant Cell Reports v.14 Screening for in vitro shoot-forming capacity of seedling explants in bell peper (Cpasicum annuum L.) genotypes and efficient plant regeneration using thidiazuron Attila S;Giuseppe N;Miklos F
  3. Plant Cell Reports v.15 Induction of direct somatic embryogenesis and plant regeneration in peper (Capsicum annuum L.) Binzel ML;Sankhla N;Joadhi S;Sankhla D
  4. Plant Cell Reports v.14 Improved flax regeneration from hypocotyls using thidiazuron as a cytokinin source Bretagne B;Chupeau M;Chupeau Y;Fouilloux G
  5. Plant Cell Reports v.15 Influence of BA and sucrose on the competence and determination of pepper (Capsicum annuum L. var. Sweet Vanana)hypocotyl cultures during shoot formation Czrl M;Leung WM
  6. Plant Molecular Biology v.26 Early flowering and reduced apical dominance results form ectopic expression of a rice MADS box gene Chung YY;An G
  7. Plant Cell Reports v.12 Efficient regeneration of plants independent of exogeneous growth regulatots in bell pepper (Capscium annuum L.). Hiroshi E;Satoshi N;Masakazn K
  8. Plant Cell Tissue and Organ Culture v.33 Thidiazuron: a potent cytokinin for woody plant tissue culture Huetteman C;Preece J
  9. Cell v.68 The homeotic gene APETAL3 of Arabidopsis thaliana encodes a MADS box and is expressed in petals and stamens Jack T;Brochman LL;Meyerowitz EM
  10. Plant Science v.115 Characterization and transcriptional profiles of two rice MADS box genes Jia HW;Chen R;Luo D
  11. Plant Cell Reports v.16 In vitro plant regeneration from leaf callus in Piper colubriuum Link Kelkar SM;Deboo GB;Krisnamurthy KV
  12. Acta Hort v.483 Regeneration and genetic transformation of hot pepper plants Lim HT;Lee GY;You YS
  13. Plant Cell Report v.16 Satellite-RNA-mediated resistance to cucumber mosaic virus in transgenic plants of hot pepper (Capsicum annuum cv. Golden Tower). Kim SJ;Lee SJ;Paek KH
  14. Plant Cell Reports v.9 Agrobacterium induced gall formation in bell pepper (Capsicum annuum L.) and foramation of shoot-like structure expressing introduced genes Liu W;Parrott WA;Willams EG
  15. Plant Cell Reports v.17 Efficient shoot organogenesis of eggplant (Solanum melongena L.) induced by thidiazuron Magioli C;Rocha APM;Oliveira DE
  16. Nature v.360 Molecular characterization of the Arabidopsis floral homeotic gene APETALAI Mandel MA;Gustafson-Brown C;Savidge B;Yanofsky MF
  17. Plant Science v.131 Agrovacterium-mediated genetic transformation in hot chilli (Capsicum anuum L. var. Pusajwala) Manoharan M;Lakshmi SG
  18. Plant Cell Reports v.15 introduction of direct somatic embryogenesis and plant regeneration in pepper (Capsicum annuum L.) Marla L;Sankhla D
  19. Scientia Hor ticulture v.42 Cultivar differences in shoot forming capacity of hypocotyl tissues of chilli pepper (Capsicum annuum L.) cultured in vitro Neftali OA;Leticia IM
  20. Plant Cell Report v.16 An improved and reliable chilipepper (Capsicum annuum L.). Rafael RM;Neftali OA
  21. Science v.250 Genetic control of flower development: Homeotic genes in Antirrhinum majus Schwarz-Sommer Z;Huijser P;Nacken W;Saedler H;Sommer H
  22. Plants v.210 Developmentally regulated expression of two MADS box genes MdMADS3 and MdMAD4, in the morphogenesis of flower buds and fruit in appl Sung SK;Yu GH;Nam JM;Jeong DH;An GH
  23. Plant Cell Report v.15 Organogenesis in Camellia×Williamsii: cytokinin requirement and susceptibility antibiotics Tosca A;Pandolfi R; Vasconi S
  24. J. Plant Biotechnology v.2 Optimised protocols for efficient plant regeneration and gene transfer in pepper (Capsium annuum L.). Virag M;Miklos F;Attila S;Ervin B;Istvan N
  25. Nature v.346 The protein encoded by the Arabidopsis homeotic gene agamous resembles transicription factor Yanofsky MF;Ma H;Bowman JI;Drews GN;Feldmann KA;Meyerowitz EM
  26. Korea J. Plant Tissue Culture v.24 Plant regeneration and expression of Mouse Adenosine Deaminase gene in transgenic hot pepper (Capsicum annuum L.) Plants Yong DC;Lee KY;Yoo YS;Kim HT
  27. Ph. D. thesis. Seoul National University Molecular isolation and characterization of three MADS genes from hot pepper plants (Capscium annuum L.) Yu JW
  28. Plant Cell Reports v.16 Transgenic sweet pepper plants from Agrobacterium mediated transformation Zhu YX;Zhang WY;Chen ZL