Korean Journal of Veterinary Service (한국동물위생학회지)
- Volume 24 Issue 4
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- Pages.335-341
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- 2001
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- 3022-7372(pISSN)
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- 2287-7630(eISSN)
Rapid detection of Brachyspira hyodysenteriae in swine intestinal specimens by PCR
- Dong-Kyun Suh (Daegu Metropolitan Health & Environment Institute) ;
- Yun-Jeong Do (College of Veterinary Medicine, Kyungpook National University) ;
- Jong-Su Ha (College of Veterinary Medicine, Kyungpook National University) ;
- Kyeong-Hyeon Lee (College of Veterinary Medicine, Kyungpook National University) ;
- Dong-Jun Song (Daegu Metropolitan Health & Environment Institute) ;
- Chun-Sik Lee (Daegu Metropolitan Health & Environment Institute) ;
- Young-Chan Bae (Daegu Metropolitan Health & Environment Institute) ;
- Suk-Chan Jung (National Veterinary Research & Quarantine Service) ;
- Won-Pil Choi (College of Veterinary Medicine, Kyungpook National University)
- Published : 2001.12.01
Abstract
Swine dysentery caused by Brachyspira hyodysenteriae, an anaerobic, beta-hemolytic spirochete, is a severe mucohemorrhahic diarrheal disease that primarily affects pigs during the growing and finishing period. The current standard laboratory procedure to culture and identify B hyodysenteriae takes 3 to 7 days. This report present a rapid PCR for detection B hyodysenteriae in a single reaction using DNA from swine intestinal samples. The PCR produced a specific 421bp PCR product with template DNA purified from B hyodysenteriae, and the accuracy for detection of B hyodysenteriae by PCR results compared with those of conventional method was 100% in intestinal specimens. Nonspecific bands were not detected with B innocens, a nonpathogenic common inhabitant spirochete, including other enteric bacterial organisms. This procedure could detect as little as 50 pg of template DNA for B hyodysenteriae.