Korean Journal of Animal Reproduction (한국가축번식학회지)
- Volume 25 Issue 4
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- Pages.359-369
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- 2001
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- 1226-5284(pISSN)
Differences by RAPD-PCR Analysis within and between Rockfish (Sebastes schlegeli) Populations from the Yellow Sea and the Southern Sea in Korea
황해 및 남해산 조피볼락 (Sebastes schlegeli) 개체군 사이의 RAPD-PCR 분석에 의한 차이
- Yoon, Jong-Man (Department of Marine Biomedical Science, College of Ocean Science and Technology, Kunsan National University) ;
- Kim, Jong-feon (Department of Marine Biomedical Science, College of Ocean Science and Technology, Kunsan National University)
- Published : 2001.12.01
Abstract
Polymerase chain reaction (PCR) amplification of DNA as 30 different arbitrary primers and random amplified polymorphic DNAs (RAPD) analysis were performed on genomic DNA extracted from the blood of the marine rockfish (Sebastes schlegeli) from the Yellow Sea and the Southern Sea. The unique properties of the genomic DNA were used to investigate the features of the population dynamics and origins of the species. Out of 30 primers, seven generated 207 highly reproducible RAPD polymorphic products, producing approximately 2.7 polymorphic bands per primer. About 67.4% of total amplified products (307) were either polymorphic (207) to rockfish. The degree of similarity varied from 0.22 to 0.63 as calculated by bandsharing analysis. Also, the average level of bandsharing was 0.39
황해와 남해산이 각각 50마리씩 총100마리의 조피볼락 (Sebastes schlegeli)의 DNA를 혈액으로부터 추출하여 30개의 무작위 primer를 사용한 RAPD(random amplified polymorphic DNAs)-PCR (polymerase chain reaction) 방법으로 분석하였다 genomic DNA의 독특한 특징들이 그 어종군의 특징을 알아내기 위해서 사용되었다. 30개의 primer 중에서 7개의 primer로 부터 증폭된 전체 산물 (307) 중에서 약 67.4%인 207개의 다형성의 산물 (polymorphic products)이 나타났고, 1개의 primer당 약 2.7개의 다양성의 산물 (polymorphic bands)이 확인되었다. 황해산의 경우 bandsharing analysis를 통해서 볼 때 0.22로부터 0.63까지의 bandsharing value가 확인되었고, 이러한 수치는 0.39