Abstract
This study was carried out to investigate the general characteristics such as volume, sperm concentration, sperm motility, sperm abnormality on whole semen, RSP-S and RSP-T semen and fractional semen of small size dogs, and the effect of temperature and preservation time and cryoproservation on motility of whole and RSP-S and RSP-T semen. Multiple ejaculates were collected from small dogs by the digital manipulation of penis. 1. The volume per ejaculate semen, sperm of concentration and motility and abnormal sperm rate of 1st fractional semen were 0.65$\pm$0.09 $m\ell$, 4.52$\pm$0.35$\times$10$^{6}$ cells/$m\ell$, 15.64$\pm$3.85% and 5.50$\pm$0.62%. Also, 2nd fractional semen were 1.25$\pm$0.20$m\ell$, 3.35$\pm$0.48$\times$10$^{6}$ cells/$m\ell$, 96.25$\pm$4.65% and 4.24$\pm$0.46%. And 3rd fractional semen were 1.45$\pm$0.21$m\ell$, 3.55$\pm$0.52$\times$10$^{6}$ cells/$m\ell$, 92.82$\pm$4.24% and 4.66 $\pm$0.58%, respectively. 2. The sperm of concentration and motility and abnormal sperm rates of whole, RSP-S and RSP-T semen were 5.45$\pm$0.82$\times$10$^{6}$ cells/$m\ell$, 95.55 $\pm$4.65%, 4.58$\pm$0.45% and 4.82$\pm$0.36$\times$10$^{6}$ cells/$m\ell$, 90.10$\pm$3.42%, 6.48$\pm$0.68% and 4.55$\pm$0.45$\times$10$^{6}$ cells/$m\ell$, 93.25$\pm$3.85%, 4.82$\pm$0.58%, respectively. 3. The motility of whole, RSP-S and RSP-T semen were higher at 4$^{\circ}C$ than at 38$^{\circ}C$. When preservation temperature was at 4$^{\circ}C$, survival rates of RSP-S and RSP-T sperm were 97.54~6.25% at 1~72 hrs, 97.40~5.62% at 1~100 hrs, respectively. 4. The survival rates of slow and rapid frozen 2nd fraction, RSP-S and RSP-T semen were 67.3$\pm$4.45%, 88.8$\pm$4.46% and 46.4$\pm$3.84%, 74.4$\pm$4.20%, respectively. Survival rates was significantly higher in frozen RSP-S and RSP-T semen than that in control group(8.5$\pm$2.12%).
본 연구는 소형 개의 동결정액을 개발하여 인공수정에 이용하고자 제 2차 분획정액을 생리적 식염수와 Iris-buffer액으로 희석하고 원심분리에 의해 정장성분을 제거한 RSP-5 및 RSP-T 정액의 일반성상과 단기보존 및 동결보존시의 생존성에 대해 조사하였다. 1, 제 1분획의 정액량은 0.65$\pm$0.09 $m\ell$, 정자농도는 4.52$\pm$0.35$\times$$10^{6}$cells/$m\ell$, 활력은 15,64$\pm$3.85%, 기형정자수는 84.36$\pm$0.62%였으며, 제 2분획의 정액량은 1.25$\pm$0.20 $m\ell$, 정자농도는 3.35$\pm$0.48$\times$$10^{6}$cells/$m\ell$, 활력은 96.25$\pm$4.65%, 기형정자수는 3.75$\pm$0.46%였으며, 제 3분획의 정액량은 1.45$\pm$0.21 $m\ell$, 정자농도는 3.85$\pm$0.52$\times$$10^{6}$cells/$m\ell$, 활력은 92.82$\pm$4.24%, 기형정자수는 4.66$\pm$0.58%였다. 2. 제 2분획 정액의 RSP-5군의 정자농도는 4.82$\pm$0.36$\times$$10^{6}$cells/$m\ell$, 활력은 90.10$\pm$3.42%, 기형정자수는 9.90$\pm$0.68%였으며, RSP-T군의 정자농도는 4.55$\pm$0.45$\times$$10^{6}$cells/$m\ell$, 활력은 93.25$\pm$3.85%, 기형정자수는 6.75$\pm$0.58%였다. 이는 대조군의 정자농도 5.45$\pm$0.82$\times$$10^{6}$cells/$m\ell$, 활력 95.55$\pm$4.65%, 기형정자수 4.45$\pm$0.45%에 비해 높게 나타났다. 3. 제 2분획 정액을 RSP-5와 RSP-T로 처리한 정액을 4$^{\circ}C$에서 보존했을 때 각각 1~72시간(97.54~6.25%)과 1~100시간(97.40~5.62%)에서 생존성이 유지되었고, 38$^{\circ}C$에서 보존했을 때 1~40시간(96.45~7.45%)과 1~50시간(95.60~B.65%)에서 생존성이 유지되었다. 이는 무처리 대조군의 1~50시간(97.24~4.82%)과 1~30시간(95.52~5.55%)에 비해 생존성이 높게 나타났다. 4. 제 2분획 정액을 처리한 RSP-5군의 정액을 완만 및 초급속 동결후 융해했을 때 생존율은 각각 67.3$\pm$4.45%와 46.4$\pm$3.84%였으며, RSP-T군 정액은 58.8$\pm$4.46%와 74.4$\pm$4.20%로서 대조군의 8.5$\pm$2.12%에 비해 높게 나타났다.