Toxicological Research

  • 제17권4호
  • /
  • Pages.241-248
  • /
  • 2001
  • /
  • 1976-8257(pISSN)
  • /
  • 2234-2753(eISSN)
한국독성학회 (Korean Society of Toxicology & Korea Environmental Mutagen Society)
권호 표지

Induction of Apoptosis in the Testes of SD Rats After Exposure to 2-Bromopropane

  • Kim, Young-Hee (Toxicology Research Center, Korea Research Institute of Chemical Technology) ;
  • Cho, Sung-Whan (Chungnam National University) ;
  • Ha, Chang-Su (Toxicology Research Center, Korea Research Institute of Chemical Technology) ;
  • Kang, Boo-Hyon (Toxicology Research Center, Korea Research Institute of Chemical Technology)
  • 발행 : 2001.12.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Exposure to 2-Bromopropane has been known to cause degeneration of male germ cells. However the mechanism underlying this process is poorly understood. The objective of this study was to determine whether or not the exposure of male Sprague-Dawley rats to 2-BP induces apoptosis in male germ cells. Male rats(N=3 or 4 in each group) were orally administered either with the corn oil vehicle (10 ml/kg body weight) or with 2-BP (3,500 mg/kg) once a day for 3 days. The presence of apoptosis was determined by TUNEL detection in situ and by an increase in DNA fragmentation. A low spontaneous incidence of apoptosis was observed in vehicle control animals, especially in pre-meiotic germ cells of stages I-VI and stages XII-XIV the seminiferous tubules. In 2-BP exposure rats, the incidence of apoptosis markedly increased at 4 h, reached a peak at 8 h (about 7-fold over control), and then decreased rapidly to control level by 48 h after the last administration. Although apoptosis induced by 2-BP occurred in all stages of germ cells, it was most pronounced in spermatogonia and early spermatocytes in stages I-VI and stages XII-XIV. Taken together, our results suggest that apoptosis is involved in the toxicity of testicular germ cells resulting in oligospermia or azoospermia after exposure to 2-BP.

키워드

참고문헌

  1. Toxical. Appl. Pharmacol v.137 Fate of germ cell in 2,5-hexanedione induced testicular injury:Ⅱ, Atrophy persists due to a reduced stem cell mass and ongiong appotosis Allard, E.K.;Boekeheide, K.
  2. Toxicol.Appl. Pharmacol. v.137 Fate of germ cell in 2,5-hexandione induced testlcular injury: I Apoptosis is the mechanism of cell death Blanchard, K.T.;Allard, K.E.;Boerkelheide, K.
  3. Cell Proltf v.29 Spontanceous germ cell death in the testis of the adult rat takes the form of apoptosis: re-evalution of cell types that exhibit the ability to die during spermatogenesis Blanco-Rodriguez, J.;Martinez-Garcia, C.
  4. J. Reprod Fertillity v.105 Identification of male germ cells undergoing apoptosis in adult rates Brinkworth, M.H.;Weinbauer, G.F.;Schlatt, S.;Nieschlag, E.
  5. Biol. Reprod. v.56 Induction of apoptosis in the germ cells of adult male rates after exposure to cycophosphamide Cai, L.;Hales, B.F.;Robaire, B.
  6. Modern Pathol. v.7 Necrosis versus Apoptosis Farber, E.
  7. Mutat Res. v.301 Effects of etoposide on stage-specific DNA synthesis during rat spermatogenesis Hakovirta, H.;Parvien, M.;lahdetie, J.
  8. Int. J. Androl. v.18 In situ quantification of stage specific apoptosis in the rat seminiferous epothelium:effects of short-term sxperimental cryptorchidism Henriksens, K.;Hakovirta, H.;Parvinen, M.
  9. Endocrinology v.136 Testosterone inhibits and induces apoptosis in rat seminiferous tubules in a stage-specific manner: in situ quantification in squash preparation after administration of ethane sulfonate Henriksen, K.;Hakovirta, H.;Parvinen, M.
  10. J. Androl. v.17 Stage specific apoptosis in the rat seminiferous epithelium:quantification of irradiation effects Henriksens, K.;Kulmala, J.;Toppari, J.;Mehrotra, K.;Parvinen, M.
  11. Am. J. Ind. Med. v.35 Occupational health survey on workers exposed to 2-bromopropane at low concentrations Ichihara, G.;Ding, X.;Yu, X.;Wu,X.;Kamijima, M.;Peng, S.;Jiang, Z.;Takeuchi, Y.
  12. Br. J. Cancer. v.26 Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics Kerr, J.F.;Wyllie, A.H.;Currie, A.R.
  13. J. Reprod Fertil. v.95 Spontaneous degeneration of germ cells in rat testis: Assessment of cell types and frequency during the spermatogenic cycle Kerr, J.B.
  14. Eur. J. Endocrinol. v.138 Hormonal control of programmed cell death/apptosis Kiess, W.;Gallaher, B.
  15. J. Androl. v.19 Quantification of apoptotic testicular germ cells in normal and methoxyacetic acid treated mice as determined by flow cytometry Krishnamurthy, H.;Weinbauer, G.F.;Aslam, H.;Yeung, C.H.;Nieschlag, E.
  16. J. Toxiciol. Pub. Health. v.14 A 4-weeks oral toxicity study of 2-bromopropane in Sprageu-Dawlsy male rats Lee, H.S.;Kang, B.H.;Son, H.Y.;Kim, H.Y.;Cho, Y.C.;Roh, J.K.
  17. Endocrinology v.140 Single exposure to hear induces stage specific germ cell apoptosis in rats:Role of intratesticular testosterone on stage specificity Lue, Y.H.;Hikim, A.P.S.;Swerdloff, R.S.;Taing, H.S.;Bui, T.;Leung, A.;Wang, C.
  18. Ind. Health v.35 Mutagenicity of 2-bromopropane Maeng, S.H.;Yu, I.J.
  19. Biol. Reprod. v.61 Germ cell apoptosis in the testes of Sprague-Dawley rats following testosterone withdrawal by ethane 1,2-demethane-sulfonate administration: relationship to Fas Nandi, S.;Banerjee, P.P.;Zirkin, B.R.
  20. Endocrinology v.135 Testosterone promotes the conversion of round spermatids between stages Ⅶ and Ⅷ of the rat spermatogenic cycle O'Donnell, L.;McLachlan, R.I.;Wreford, N.G.;Robertson, D.M.
  21. Toxicol. Lett. v.104 Histopathological evidence that spermatogonia are the target cells of 2-brompropane Omura, M.;Romero, Y.;Zhao, M.;Inoue, N.
  22. Toxicol. Appl. Pharmacol v.137 Mono-{2-ethyl-hexy} phthalate rapidly alters both Sertoll cell vimenthin filaments and germ cell apoptosis in young rat testes Richburg, J.H.;Boekelheide, K.
  23. Toxicol. Appl. Pharmacol v.128 Seminiferous tubule fluid sercretion is a Sertoli cell microtubule dependent process inhibited by 2,5-hexanedione exposure Richburg, J.H.;Redenbach, D.M.;Boerkelheide, K.
  24. Cache River Press: Clearwater FI UAS Histological and histopathological evaluation of the testis Russell, L.D.;Ettlin, R.A.;Sinha hikim, A.P.;Clegg, E.D.
  25. Kor. J. Vet. Pathol. v.1 Testicular lesion in the Sprageu-Dawley rats treated with high dose of 2-bromopropane Son, H.Y.;Cho, S.W.;Kim, Y.B.;Ha, C.S.;kang, B.H.
  26. J. Toxicol. Pub. Health v.14 Histopathological observation and flow cytometry analysis of testicular atrophy induced by 2-bromopropane on the Sprague-Dawley rat Son, H.Y.;Kang, B.H.;Cho, S.W.;Cha, S.W.;Roh, J.K.
  27. Reprod Toxicol. v.13 Effect of 2-bromopropane on spermatogenesis in Sprague-Dawley rats SOn. H.Y.;Kim, Y.B.;Kang, B.H.;Cho, S.W.;Ha, C.S.;Roh, J.K.
  28. Herz. v.24 Morphologic criteria and detection of apoptosis Saraste, A.
  29. Toxicol. Appl. Pharmacol. v.155 Involvement of germ cell apoptosis un the induction of testicular toxicity following hydroxyurea treatment Shin, J.H.;Mori, C.;Shiota, K.
  30. Environ Mol. Mutagen v.31 Apoptotic response of spermatogenic cells to the germ cell mutagens etoposide, adriamycin, and diepoxybutane Sjoblom, T.;West, A.;Lohdetie, J.
  31. Reprod Toxicol. v.12 Germ cell apoptosis in rat testis after administration of 1,3-dini-trobenzene Strandgaard, C.;Miller, M.C.
  32. Science v.267 Apoptosis in the pathogenesis and treatment of disease Thompson, C.B.
  33. Mol. Hum. Reprod. v.4 Apoptosis and expression of apoptotic regulators in the human testic followinf short and long term anti-androgen treatment Woolveridge, I.;Bryden, A.A.G.;Taylor, M.F.;George, N.J.R.;Wu, F.C.W.;Morris, I.D.
  34. Scand J. Environ. Health. v.23 Reproductive toxicity of 2-bromopropane Yu, I.J.;Chung, Y.H.;Lim, C.H.;Maeng, S.H.;Lee, J.Y.;Kim, H.Y.;Lee, S. J.;Kim, C.H.;Kim. T.K.;Lim, C.H.;Park, J.S.;Moon, YH.