Animal cells and systems

The Korean Society for Integrative Biology (한국통합생물학회)
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Identification and Phylogenetic Analysis of SINE-R Retroposon Family in cDNA Library of Human Fetal Brain

  • Yi, Joo-Mi (Division of Biological Sciences, College of Natural Sciences, Pusan National University) ;
  • Shin, Kyung-Mi (Division of Biological Sciences, College of Natural Sciences, Pusan National University) ;
  • Lee, Ji-Won (Division of Biological Sciences, College of Natural Sciences, Pusan National University) ;
  • Paik, In-Ho (Department of Psychiatry, College of Medicine, The Catholic University of Korea) ;
  • Jang, Kyung-Lib (Division of Biological Sciences, College of Natural Sciences, Pusan National University) ;
  • Kim, Heui-Soo (Division of Biological Sciences, College of Natural Sciences, Pusan National University)
  • Published : 2001.09.01
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Abstract

SINE-R retroposons have been derived from human endogenous retrovirus HERV-K family and found to be hominoid specific. Both SINE-R retroposons and HERV-K family are potentially capable of affecting the expression of closely located genes. From cDNA library of human fetal brain, we identified seven SINE-R retroposons and compared them with sequences derived from GenBank database. The SINE-R retroposons from human feta1 brain showed 85∼97% sequence similarities with the human-specific retroposon SINE-R.C2. They also showed 88∼96% sequence similarities with the sequence of the schizo-cDNA clone that derived from postmortem frontal cortex tissue of a schizophrenic patient. Phylogenetic analysis using the neiqhbor-joining method revealed that the seven new SINE-R retroposons from cDNA library of the human feta1 brain have proliferated independently during human evolution. The data indicate that such SINE-R retroposons are expressed in human fetal brain and deserve further investigation as potential leads to understanding of neuropsychiatric diseases.

Keywords

References

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