Animal cells and systems

  • 제5권3호
  • /
  • Pages.217-224
  • /
  • 2001
  • /
  • 1976-8354(pISSN)
  • /
  • 2151-2485(eISSN)
한국통합생물학회 (The Korean Society for Integrative Biology)
권호 표지

Expression and Regulation of Gonadotropin-Releasing Hormone(GnRH) and Its Receptor mRNA Transcripts During the Mouse Ovarian Development

  • Shim, Chanseob (School of Biological Sciences, Seoul National University) ;
  • Khang, Inkoo (School of Biological Sciences, Seoul National University) ;
  • Lee, Kyung-Ah (College of Medicine, Pochon Cha University, and Infertility Medical Center, Cha General Hospital) ;
  • Kim, Kyungjin (College of Medicine, Pochon Cha University, and Infertility Medical Center, Cha General Hospital)
  • 발행 : 2001.09.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The present study examines the expression and regulation of gonadotropin-releasing hormone (GnRH) and its receptor (GnRH-R) mRNA levels during mouse ovarian development. A fully processed, mature GnRH mRNA together with intron-containing primary transcripts was expressed in the immature mouse ovary as determined by Northern blot analysis and reverse transcription-polymerase chain reaction (RT-PCR). The size of ovarian GnRH mRNA was similar to that of hypothalamus, but its amount was much lower than that in the hypothalamus. Quantitative RT-PCR procedure also revealed the expression of GnRH-R mRNA in the ovary, but the estimated amount was a thousand-fold lower than that in the pituitary gland. We also examined the regulation of ovarian GnRH and GnRH-R mRNA levels during the follicular development induced by pregnant mare's serum gonadotropin (PMSG) and/or human chorionic gonadotropin (hCG). Ovarian luteinizing hormone receptor (LH-R) mRNA was abruptly increased st 48 h after the PMSG administration and rapidly decreased to the basal level thereafter. Ovarian GnRH mRNA level was slightly decreased at 48 h after the PMSG administration, and then returned to the basal value. GnRH-R mRNA level began to increase at 24 h after the PMSG treatment, decreased below the uninduced basal level at 48 h, and gradually increased thereafter. HCG administration did not alter ovarian GnRH mRNA level, while it blocked the PMSG-induced increase in GnRH mRNA level. Taken together, the present study demonstrates that the expression of GnRH and GnRH-R mRNA are regulated by gonadotropin during follicular development, suggesting possible intragonadal paracrine roles of GnRH and GnRH-R in the mouse ovarian development.

키워드

참고문헌

  1. Aatsinki JT, Pietila EM, Lakkakorpi JT, and Rajaniemi HJ (1992) Expression of the LH/hCG receptor gene in rat ovarian tissue is regulated by an extensive alternative splicing of the primary transcript. Mol Cell Endocrinol 84: 127-135 https://doi.org/10.1016/0303-7207(92)90079-L
  2. Bacich DJ, Rohan RM, Norman RJ, and Rogers RJ (1994) Characterization and relative abundance of alternatively spliced luteinizing hormone receptor messenger ribonucleic acid in the ovine ovary. Endocrinology 135: 735-744 https://doi.org/10.1210/en.135.2.735
  3. Bauer-Dantoin AC and Jameson JL (1995) Gonadotropin-releasing hormone receptor messenger ribonucleic acid expression in the ovary during the rat estrous cycle. Endocrinology 136: 4432-4438 https://doi.org/10.1210/en.136.10.4432
  4. Billig H, Furata I, and Hsueh AJW (1994) Gonadotropin-releasing hormone directly induces apoptotic cell death in the rat ovary: Biochemical and in situ detection of deoxyribonucleic acid fragmentation in graulosa cells. Endocrinology 134: 245-252 https://doi.org/10.1210/en.134.1.245
  5. Camp TA, Rahal JO, and Mayo KE (1991) Cellular localization and hormonal regulation of follicle-stimulating hormone and luteinizing hormone receptor messenger RNAs in the rat ovary. Mol Endocinol 5: 1405-1417
  6. Chomczynski P and Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloro-form extraction. Anal Biochem 162: 156-159
  7. Clayton RN (1989) Gonadotropin-releasing hormone: its actions and receptors. J Endocrinol 120: 11-19
  8. Clayton RN, Eccleston L, Gossard F, Rhalbard JC, and Morel G (1992) Rat granulosa cells express the gonadotropin-releasing hormone gene: envidence from in situ hybridization. J Mol Endocrinol 9: 189-195
  9. Ekholm C, Hillensjo T, and lsaksson O (1981) Gonadotropin-releasing hormone agonists stimulate oocyte meiosis and ovulation in hypophysectomized rats. Endocrinology 108: 2022-2024
  10. Goubau S, Bond CT, Adelman JP, Misra V, Hynes MF, Schultz GA, and Murphy BD (1992) Partial characterization of the gonadotropin-releasing hormone (GnRH) gene transcript in the rat ovary. Endocrinology 130: 3098-3100 https://doi.org/10.1210/en.130.5.3098
  11. Hillensjo T and LeMaire WJ (1980) Gonadotropin-releasing hormone agonists stimulate meiotic maturation of follicle-enclosed rat oocytes in vitro. Nature 287: 145-146 https://doi.org/10.1038/287145a0
  12. Hogan B, Costantini F, and Lacy E (1994) Manipulating the Mouse Embryo. A Laboratory Manual. Cold Spring Harbor Laboratory, New York, pp 130-132
  13. Kakar SS and Jennes L (1995) Expression of Gonadotropin-releasing hormone and Gonadotropin-releasing hormone receptor mRNAs in various non-reproductive human tissue. Cancer Lett 98: 57-62 https://doi.org/10.1016/0304-3835(95)90203-1
  14. Mason AJ, Hayflick JS, Zoeller RT, Young WSIII, Pillips HS, Nikolics K, and Seeburg PH (1986) A deletion truncating the Gonadotropin-releasing hormone gene is responsible for hypogonadism in the hpg mouse. Science 234: 1366-1371 https://doi.org/10.1126/science.3024317
  15. Minaretzis D, Jakubowski M, Mortola JF, and Pavlou SN (1995) Gonadotropin-releasing hormone receptor gene expression in human ovary and granulosa-lutein cells. J Clin Endocrinol Metab 80: 430-434 https://doi.org/10.1210/jc.80.2.430
  16. Nakamura K, Minegishi T, Takakura Y, Miyamoto K, Hasegawa Y, Ibuki Y, and lgarashi M (1991) Hormonal regulation of gonadotropin receptor mRNA in rat ovary during follicular growth and luteinization. Mol Cell Endocrinol 82: 259-263 https://doi.org/10.1016/0303-7207(91)90039-U
  17. Oikawa M, Dargan C, Ny T, and Hsueh AJW (1990) Expression of Gonadotropin-releasing hormone and prothymosin-a messenger ribonucleic acid in the ovary. Endocrinology 127: 2350-2356
  18. Park Y, Kam K, Cheon M, and Ryu K (2001) Regulation of Gonadotropin-releasing hormone receptor mRNA expression on cultured rat granulosa cells. Yonsei Med J 42: 233-241
  19. Peng XR, Hsueh AJ, LaPolt PS, Bjersing L, and Ny T (1991) Localization of luteinzing hormone follicle development and ovulation. Endocrinology 129: 3200-3207
  20. Peng C, Fan NC, Ligier M, Vaananen J, and Leung PCK (1994) Expression and regulation of Gonadotropin-releasing hormone (GnRH) and GnRH receptor messenger ribonucleic acids in human granulosa-luteal cells. Endocrinology 135: 1740-1746 https://doi.org/10.1210/en.135.5.1740
  21. Reinhart J, Mertz LM, and Catt KJ (1992) Molecular cloning and expression of cDNA encoding the murine Gonadotropin-releasing hormone receptors. J Biol Chem 267: 21281-21284
  22. Sambrook J, Frisch EF, and Maniatis T (1989) Molecular coloning: A Laboratory Manual. Cold Spring Harbor Laboratory, New York, pp. 9.31-9.57
  23. Seong JY, Park S, and Kim K (1999) Enhanced splicing of the first intron from the Gonadotropin-releasing hormone(GnRH) primary transcript is a prerequisite for mature GnRH messenger RNA: presence of GnRH neuron-specific splicing factors. Mol Endocrinol 13: 1882-1895 https://doi.org/10.1210/me.13.11.1882
  24. Shim C, Kwon HB, and Kim K (1996) Differential expression of laminin chain-specfic mRNA transcripts during mouse preim-plantation embryo development. Mol Reprod Dev 44: 44-55 https://doi.org/10.1002/(SICI)1098-2795(199605)44:1<44::AID-MRD5>3.0.CO;2-T
  25. Shim C, Choi D, Kwon HB, and Kim K (1997) Expression of laminin chain-specfic gene transcripts in mouse uterine tissues during peri-implantation period. Mol Reprod Dev 48: 176-184 https://doi.org/10.1002/(SICI)1098-2795(199710)48:2<176::AID-MRD5>3.0.CO;2-P
  26. Stojilkovic SS, Reinhart J, and Catt KJ (1994) Gonadotropinreleasing hormone: Structure and signal transduction pathways. Endocr Rev 15: 462-499 https://doi.org/10.1210/er.15.4.462
  27. Tsai-Morris CH, Buczko E, Wang W, Xie XZ, and Dufau ML (1991) Structural organization of the rat luteinizing hormone(LH) receptor gene. J Biol Chem 266: 11355-11359
  28. Whitelaw PE, Eidne A, Sellar R, Smyth CD, and Hillier SG (1995) Gonadotropin-releasing hormone receptor messenger ribonucleic acid expression in rat ovary. Endocrinology 136: 172-179 https://doi.org/10.1210/en.136.1.172
  29. Wolfahrt S, Kleine B, Jerry H, and Rossmanith WG (2001) Endogenous regulation of the GnRH receptor by GnRH in the human placenta. Mol Human Reprod 17: 89-95 https://doi.org/10.1093/molehr/7.1.89
  30. Yoshimura Y, Nakamura Y, Yamada H, Nanno T, Ubukata Y, Ando M, and Suzuki M(1991) Gonadotropin-releasing hormone agonists induce meiotic maturation and degeneration of oocytes in the in vitro perfused rabbit ovary. Fertl Steril 55: 177-183