Decrease of c-Fos Expression in Hippocampus of Anorexia(anx/anx) Mice

  • Kim, Soon Ae (Department of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Choi, Young Mee (Department of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Park, Hi-Joon (Department of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Lee, Hyangsook (Department of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Han, Jin A (Department of Medical Nutrition, Graduate School of East-West Medical Science, Department of Biology, College of Art and Science, Kyung Hee University) ;
  • Kang, Soon Ah (Department of Medical Nutrition, Graduate School of East-West Medical Science, Department of Biology, College of Art and Science, Kyung Hee University) ;
  • Choue, Ryo Won (Department of Medical Nutrition, Graduate School of East-West Medical Science, Department of Biology, College of Art and Science, Kyung Hee University) ;
  • KimKwon, Yunhee (Department of Biology, College of Art and Science, Kyung Hee University) ;
  • Kim, Chang-Ju (Kohwang Medical Research Institute, Department of Pharmacology, College of Medicine, Kyung Hee University) ;
  • Chung, Joo-Ho (Department of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung Hee University)
  • 발행 : 2001.06.01

초록

Mice homozygous for the lethal autosomal recessive anorexia mutation (anx) present with premature death around postnatal day 22. The anorexia mutant mice also present phenotypes such as reduced body weight, decreased food intake, and abnormal behavior characteristics such as body tremors, hyperactivity, uncoordinated gait, and head weaving. In order to investigate the expression of c-Fos in the hippocampus of anorexia mutant mice, the immunohistochemistry was performed in this study. The anorexia mutant mice exhibited lower expression of c-Fos in the hippocampus regions thBn the control group. In the CA3 and dentate gyrus, the number of c-Fos-positive cells in anorexia mutant mice was noticeably lower than that in control mice. However, no significant difference was found in the number of c-Fos-positive cells in CA1 of the two groups. The result suggests that the phenotypic characteristics of anorexia mutant mice may be associated with the hippocampal deficits of c-Fos expression.

키워드

참고문헌

  1. American Psychiayric Association (1994) Diagnostic and Statistical Manual of mental Disordrs DSM-IV. American Psychiatric Press, Washington DC
  2. Bendotti C, Borsini F, Cotecchia S, De Blasi A, Mennini T and Samanin R (1982) d-Amphetamine-induced anorexia and motor behavior after chronic treatment in rats: relationship with change in the number of catecholamine receptor sites in the brain. Arch Int Pharmacodyn Ther 260: 36-49
  3. Bruins-Slot L, Gorwood P, Bouvard M, Blot P, Ades J, Feingold J, Schwartz JC and Mouren-Simeoni MC (1998) Lack of association between anorexia nervosa and D3 dopamine receptor gene. Biol Psychiatry 43: 76-78 https://doi.org/10.1016/S0006-3223(97)00199-6
  4. Collier DA, Arranz MJ, Li T, Mupita D, Brown N and Treasure J (1997) Association between 5-HT2A gene promoter polymorphism and anorexia nervosa. Lancet 350: 412 https://doi.org/10.1016/S0140-6736(05)64135-9
  5. Curran T, MacConnell WP, van Straaten F and Verma IM (1983) Structure of the FBJ murine osteosarcoma virus genome;molecular cloning of its associated helper virus and the cellular homolog of the v-fos gene from mouse and human cell. Mol Cell Biol 3: 914-921
  6. Curran T, Miller AD, Zokas L and Verma IM (1984) Viral and cellular fos proteins;a comparative analysis. Cell 36: 259-268 https://doi.org/10.1016/0092-8674(84)90219-8
  7. Curran T and Teich NM (1982) Identification of a 39,000-dalton protein in cells transformed by the FBJ murine osteosarcoma virus. Virology 116: 221-235 https://doi.org/10.1016/0042-6822(82)90415-9
  8. Currie PJ, Coscina DV and Fletcher PJ (1998) Reversal of fenfluramine and fluoxetine anorexia by 8-OH-DPAT is attenuated following raphe injection of 5,7-dihydroxytryptamine. Brain Res 800: 62-68 https://doi.org/10.1016/S0006-8993(98)00497-1
  9. Franklin KBJ and Paxinos G (1997) The Mouse Brain in Stereotaxic Coordinates. Academic Press, San Diego
  10. Gorwood P, Bouvard M, Mouren-Simeoni MC, Kipman A and Ades J (1998) Genetics and anorexia nervosa: a review of candidate genes. Psychiatr Genet 8: 1-12
  11. Herdegen T and Leah JD (1998) Inducible and constitutive transcription factors in the mammalian nervous system;control of gene expression by Jun, Fos and Krox, and CREB/ATF proteins. Brain Res Rev 28: 370-490 https://doi.org/10.1016/S0165-0173(98)00018-6
  12. Hoek HW (1991) The oncidence and prevalence of anorexia nervosa and bulimia nervosa in primary care. Pshychol Med 21: 455-460
  13. Holland AJ, Sicotte N and Treasure J (1988) Anorexia nervosa;evidence for a genetic basis. J psychosom Res 32: 561-571 https://doi.org/10.1016/0022-3999(88)90004-9
  14. Huang XF and Wang H (1998) Altered c-fos expression in autonomic regulatory centers of genetically obese (ob/ob) mouse brain. Brain Res 799: 307-310 https://doi.org/10.1016/S0006-8993(98)00541-1
  15. Huh Y, Kim C, Lee W, Kim J and Ahn H (1997) Age-related change in the neuropeptide Y and Nadph-diaphorasepositive neurons in the cerebral cortex and striatum of aged rats. Neurosci Lett 223: 157-160 https://doi.org/10.1016/S0304-3940(97)13430-9
  16. Jang JW, Houpt TA, Kim SJ, Joh TH and Son JH (1998) Neuropeptide Y mRNA and serotonin innervation in the arcuate nucles of anorexia mutant mice. Brain Res 790: 67-73 https://doi.org/10.1016/S0006-8993(98)00049-3
  17. Jensen FE, Firkusny IR, and Mower GD (1993) Differences in c-fos immunoreactivity due to age and mode of seizure induvtion. Mol Crains Res 17: 185-193 https://doi.org/10.1016/0169-328X(93)90001-6
  18. Joergensen J (1992) The epidemiology of eating disorders in Fyn County. Denmark, 1977-1986. Acta Psychiatr Scand 85: 30-34 https://doi.org/10.1111/j.1600-0447.1992.tb01438.x
  19. Kasof GM, Mandelzys A, Maika SD, Hammer RE, Curran T and Morgan JI (1995) Kainic acid-induced neuronal death is associated with DNA damage and a unique immediate-early gene response in c-fos-lacZ transgenic rats. J neurosci 15: 4238-4249
  20. Kerppola TK, Luk D and Curran T (1993) Fos is a preferential target of glucocorticoid receptor inhibition of AP-1 activity in vitro. Mol Cell Biol 13: 3782-3791
  21. Kim MJ, Kim Y, Kim SA, Lee HJ, Choe BK, Nam M, Kim BS, Kim JW, Yim S, Kim C and Chung JH (2001) Increases in cell prolideration and apoptosis in dentate gyrus of anorexia(anx/anx) mice. Neurosci Lett 302: 109-112 https://doi.org/10.1016/S0304-3940(01)01684-6
  22. Rutherfold J, McGuffin P, Katz RJ and Murray RM (1993) Genetic influences on eating attitudes in a normal female twin population. Psychol Med 23: 425-436
  23. Smeyne RJ, Schilling K, Robertson L, Luk D, Oberdick J, Curran T and Morgan JI (1992) Fos-lacZ transgenic mice;mapping sutes of gene induction in the central nervous system. Neuron 8: 13-23 https://doi.org/10.1016/0896-6273(92)90105-M
  24. Son JH, Baker H, Park DH, and Joh TH (1994) Drastic and selective hyperinnervation of central serotonergic neurons in a lethal neurodevelopmental mouse mutant, Anorexia (anx). Mol Brain Res 25: 129-134 https://doi.org/10.1016/0169-328X(94)90287-9
  25. Stern SL, Dixon KN, Sansone RA, Lake MD, Nemzer E and Jones D (1992) Psychoactive substance use disorder in relatives of patients with nervosa. Compr Psychiatry 33: 207-212 https://doi.org/10.1016/0010-440X(92)90032-L
  26. Stoving RK, Hangaard J, Hansen-Nord M and Hagen C (1999) A review of endocrine changes in anorexia nervosa. J Psychiatr Res 33: 139-152 https://doi.org/10.1016/S0022-3956(98)00049-1
  27. Zhang XK, Willis KN, Husmann M, Hermann T and Pfahl M (1991) Novel pathway for throid hormone receprtor action through interaction with jun and fos oncogene activties. Mol Cell Biol 11: 6016-6025