벗굴, Ostrea denselamellosa, 유생의 인공대량사육과 채묘방법에 따른 채묘율

Artificial Mass Culture of Flat Oyster Larvae, Ostrea denselamellosa, and Collection Rates according to Various Spat Collection Methods

  • 양문호 (국립수산진흥원 남해수산연구소) ;
  • 김형섭 (군산지방해양수산청 부안수산기술관리소) ;
  • 이재용 (동의대학교 생물학과) ;
  • 한창희 (동의대학교 생물학과)
  • 발행 : 2001.06.01

초록

벗굴의 종묘확보 방안을 모색하기 위하여 유생의 대량사육에 의한 인공채묘와 자연채묘율을 조사한 결과는 다음과 같다. 모패에서 방출된 D형 유생 (각장 153.4 $\mu\textrm{m}$, 각고 137.2 $\mu\textrm{m}$)을 3 톤짜리 F.R.R 수조에 5.8 개체/ml의 밀도로 대량 사육한 결과 유생사육 16일 째에 벗굴 유생의 성장률은 각장과 각고가 202.6%, 212.9%로 성장하여 310.9 $\pm$ 9.8 $\mu\textrm{m}$ ,292.2 $\pm$ 9.1 $\mu\textrm{m}$이었으며, 부착시기인 사육 20일 째는227.1%, 241.8%가 성장하여 348.4 $\pm$ 9.1 $\mu\textrm{m}$, 331.7 $\pm$ 9.4 $\mu\textrm{m}$였다. 그리고 16일 째에는 사육 기간 중 일간 사망률이 가장 높은 0.160, 일간생존율은 0.840을 보이면서 생존율은 54.8%였으며, 사육 20일 째의 최종 생존율은 43.2%였다. 굴 패각, 가리비 패각, 피조개 패각 그리고 PVC 조각을 이용한 부착기질별의 인공 채묘율은 32.9%, 24.1%, 16.8%,10.0%로 굴 패각이 32.9% (131.9 개체/패각) 로 가장 높았으며, 단위면적당 부착율도 굴 패각이 2.69 $\pm$ 0.31 개체/$\textrm{cm}^2$로 가장 높게 나타났다. 채묘방법에 따른 치패 부착율은 채묘기질을 수중에 시설한 수하식방법에서 54.2 개체/패각 (1.00 개체/$\textrm{cm}^2$) 보다 패각을 수조저면에 배열하는 바닥식이 83.8 개체/패각 (평균 1.69 개체/$\textrm{cm}^2$) 으로 높은 부착율을 보였다. 자연채묘는 노출보다는 비노출이, 채묘기질은 그물 등 부드러운 기질보다는 견고한 조개 패각을 이용하여 채묘 하는 경우가 높게 나타났으며, 수층별의 자연채묘는 표층보다는 저층에서 부착율이 높게 나타났다. 벗굴의 인공치 패는 부착 후 26일경에 각장 2.38 $\pm$ 0.97 mm, 각고 2.16 $\pm$ 0.86 mm로 성장하였고, 일간 성장률은 각장 3.75-7.69%, 각고 3.23-7.97%로 나타났으며 3개월 후 각각 28.58 $\pm$ 2.39 mm, 31.65 $\pm$ 2.03 mm로 성장하였다. 채표기질에 따른 부착율은 굴 패각, 가리비패각, 피조개패각 그리고 PVC조각에서 각각 10.3, 5.8, 4.0, 1.5개체로 굴 패각에서 가장 높게 나타났다.

This research was conducted to develop seedling production techniques in flat oyster, Ostrea dense lamellosa. The cultivation of larvae, artificial spat and spats collection in natural conditions rate were examined. In the mass culture tank (3 tons), average growth rates of the D Shape larvae of initial shell length (153.4 ㎛) and shell height (153.4 ㎛) were 202.6% and 212.9% at 16 days and 227.1%, 241.8% at 20 days, respectively. Instantaneous death and survival rate of the larvae were 0.160 and 54.8% at 16 days and 0.057 and 43.2% at 20 days, respectively. Collection rate of flat oyster from bottom using various collectors with oyster shell, scallop shell, ark shell and PVC plates were 32.9%, 24.1%, 16.8% and 10.0%, respectively. and the greatest collecting rate was 131.9 individuals/shell (32.9%) in laid collectors on the bottom. The collecting rate of the oyster larvae were better in laid collectors on the bottom 83.8 individuals/collection than in the suspended string 54.2 individuals/collection. in all collecting substrates. Early spats settled on collectors were grew to 2.38 $\pm$ 0.97 ㎜ in shell length and 2.16 $\pm$ 0.86 ㎜ In shell height at 26 days. The spats were grew to 28.58 $\pm$ 2.39 ㎜ in shell length and 31.65 $\pm$ 2.03 ㎜ in shell height during the 4 month mid-term cultivation. In the period of cultivation, the mean number of spats attached to collectors were 10.3 individuals at oyster shells, 5.8 scallop shells, 4.0 ark shells and 1.5 PVC plates, respectively.

키워드

참고문헌

  1. Bull. Korean Fish. Soc.[in Korean] v.5 no.1 Growth of transplanted portugal and olympia oystersi in the Korean coastal waters. Bae, G.M.;Bae, P.A.
  2. Bull. Korean Fish. Soc.[in Korean] v.18 no.2 Development of oyster culture industry in Korea Bae, S.W.
  3. Gamete Research v.24 Role of speratozeugmenta in the spawning ecology of the brooding oyster Ostrea edulis Foighill, D.O.
  4. Aquaculture v.2 The importance of the gatchery rearing of Pacific oyster larvae Helm, M.M.;Spencer, B.E.
  5. Aquaculture v.92 Cold storage effects on setting of larvae of Sydney rock oyster Saccostrea commericialis, and the Pacific oyster, Crassostrea gigas Holiday, J.E.;Allan, G.L.;Frances, J.
  6. Aquaculture v.97 no.4 The characterization of Ostrea denselamellosa (Mollusca, Bivalvia) chromosomes: Karyotype constitutive hetero-chromatin and nucleolus organizer regions Insua, A.;Thiriot, C.
  7. Biological Bulletin v.122 no.1 Gamatogenesis and spawning of the European oyster, Ostrea. edulis. in water if marine Loosanoff, V.L.
  8. Bull. National. Fish. Res. Dev. Inst[in Korean] v.57 Studies on the artificial spat colliction method for the pacific oyster, Crassostrea gigas (Thunberg) Min, K.S.;Kim, T.I.;Hur, S.B.;Hur, Y.B.;Park, D.W.;H.Y. Lee;Hwang M.S.
  9. Ph. D. Thesis[in Korean] Studies on the commercial scale production of artificial seedling of the pacific oyster, Crassostrea gigas (Thunberg) Min, K.S.
  10. Aquaculture v.123 no.2 Effects of nutrition on larvae production in the European oyster, Ostrea edulis Millician, P.F.;Helm, M.M.
  11. J. Mar. Biol. v.14 Observation and experiments on sex-change in the European oyster, Ostrea edulis Orton, J.H.
  12. Aquculture v.97 The Iceland scallop. Chlamys islandica (O.F. Muller) in Breidafijourdur, west Iceland. I. Spat colletion and growth during the first year Thorarinsdottir, G.G.
  13. Ph. D. Thesis[in Korean] The Biology and Seed Production of flat oyster, Ostrea denselamellosa Yang, M.H.
  14. Kor. J. Malacol. v.15 no.2 Environment characteristics of national conditions of flat oyster, Ostrea denselamellosa in Haechang Bay Yang, M.H.;Choi, S.D.;Kim, H.S.;Han, C.H.
  15. Kor. J. Malacol.[in Korean] v.15 no.2 The development of larvae and egg of flat oyster, Ostrea denselamellosa Yang, M.H.;Choi, S.D.;Kim H.S.;Han, C.H.
  16. Bull. Korean Fish. Soc.[in Korean] v.21 no.4 Improvement of the seed production method of the pen shell - The occurrence of larvae and the early growth of the spat Yoo, H.S.;Lim K.S.;Ryu, H.Y.
  17. Bull. Korean. Fish. Soc.[in Korean] v.7 no.2 Spat collection and the growth of Anadara broughtoni Schrenck Yoo, M.S.;Yoo, S.K.
  18. Kor. J. Malacol.[in Korean] v.11 no.2 Influence of water temperature and salinity on egg development larvae rearing of oyster, Crassostrea rivularis Yoo, S.K.;Kang, K.H.
  19. J. Aquacult.[in Korean] v.10 no.2 Culture condition and larval growth of the oyster, Crassostrea nippona Yoo. S.K.;Park, H.G.
  20. Biostatistical analysis(2nd ed.) Zar, J.H.