The Korean Journal of Malacology (한국패류학회지)

The Malacological Society of Korea (한국패류학회)
권호 표지

Genetic Relationship among Three Scallop Species, Chlamys farreri farreri, Patinopecten yessoensis and Agropecten irradians, Using RAPD Markers

RAPD표지인자를 이용한 3종의 가리비에 대한 유전적 유연관계

  • 지희윤 (건양대학교 의학과 생물학교실) ;
  • 김윤경 (건양대학교 의학과 생물학교실) ;
  • 박영재 (서해 수산연구소 증식과)
  • Published : 2001.06.01
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Abstract

The genetic relationship was examined with PCR-RAPD markers among three scallop species, Chlamys farreri farreri, Patinopecten yessoensis, and Agropecten irradians. Six primers were selected from 60 primers used to compare PCR-RAPD profiles among species. All primers showed distinct RAPD band patterns between the three species. In Chiamys farreri farreri, the morphological characteristics such as shell size and color were considerably different between the two geographical populations. RAPD profile, however, showed that no significant genetic differences were found between the two geographical populations. Polymorphic alleles were observed within a population of each species. Thus, PCR-RAPD markers are useful in identifying scallop species and in understanding scallop population genetic structure.

PCR-RAPD 표지인자를 이용하여 비단가리비와 큰가리비 그리고 해만가리비의 유전적 유연관계를 조사하였다. 60개의 random primer중 6개의 primer를 선택하여 시료들의 RAPD 양상을 비교하였다. 모든 primer들은 서로 다른 속의 가리비 종류에 대하여 특이적 RAPD band 형태를 나타내었다. 비단가리비에서는 패각 크기와 색깔의 특성이 두 집단간의 현저한 차이를 나타내었다. 그러나 RAPD 양상은 두 지리적 집단간에 유전적 차이를 보이지 않았다. 다형성 RAPD 대립인자들이 각 가리비종의 동일 집단에서 관찰되었다. 그러므로 RAPD 표지인자는 가리비를 동정하고 가리비 집단 구조를 연구하는데 유용하다고 사료된다.

Keywords

References

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