YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Effects of Acute Oral Administration of Bisphenol A on the Immune Function in Mice

Bisphenol A의 급성노출이 마우스의 면역기능에 미치는 영향

  • 표명윤 (숙명여자대학교 약학대학) ;
  • 변정아 (숙명여자대학교 약학대학)
  • Published : 2001.02.01
Download PDF
⟨ Previous Next ⟩

Abstract

In order to investigate the effects of bisphenol A (BPA) on immune system in mice we examined the various immunological parameters. After single oral administration of BPA to female ICR mice, the weights of bodies and lymphoid organs, splenic cellularity and hematological parameters were examined on day 2 and 7. Among them WBC and splenic cellularity were slightly decreased on day 2. To assess the effects of BPA on humoral immune responses, splenic IgM plaque forming cell (PFC) and serum IgM were assayed. When BPA was administered after immunization with SRBC, but not before immunization, IgM PFC against SRBC was significantly lowered in a dose dependent manner. Serum IgM level was also decreased on day 4 when high dose (2000 mg/kg) of BPA was administrated after injection of OVA-antigen. The indexes of splenocyte proliferation (SP) to concanavalin A (Con A) and bacterial lipopolysaccharide (LPS) were measured in vitro by MTT assay. At low concentration BPA slightly increased splenocyte proliferation but at higher concentration it showed significant inhibitory effects on cell proliferation. Mitogen-stimulated SP was also determined with spleen cells from BPA treated mice. Con A-induced SP was slightly decreased and LPS-induced SP was especially inhibited at 1000 mg/kg and 2000 mg/kg of BPA. These results indicate that BPA is able to acutly evoke humoral and cell mediated immune suppression in mice.

Keywords

References

  1. Endocrinology v.132 no.6 An estrogenic substance is released from polycarbonate flasks during autoclaving Krishnan, A. V., Stathis, O. Permuth, S. F., Tokes, L., Feldman, D., Bisphenol A
  2. Environ health Perspect v.103 no.6 Xenoestrogens Released from lacquer Coating in Food Cans Brotons, J. a., Olea-Aerrano, M. F., Villalobos, M., Pedraza, V;Olea, N
  3. Food Additives and Contaminants v.14 no.6-7 Investigations into the potential degradation of polycarbonate baby bottle during sterilization with consequent release of bisphenol A Mountfort, K. A., Kelly , J., Jickells, S. M;Castle, L
  4. Int. J. Cancer v.75 Bisphenol-A induces cellular tansformation, aneuploidy and DNA adduct formation in cultured syrian hamster embryo cells Tsutsui, T., Tamura, Y., Yagi, E.,Hasegawa, K., Takahashi, M., Maizumi, N., Yamaguchi, F;Barrett, J. C
  5. Fundamental and Appled Toxicology v.8 The developmental toxicity of bisphenol A in rats and mice Morrissey, R. E., George, J. D., Price, C. J., Tyl, R. W., Marr, M. Cl;Kimmel, C. A
  6. Immunotoxicity of metals and immunotoxicology Dayan, A.D., Hertel, R.F., Heseltine, E., Kazantzis, G., Smith, E.M;Van der Venne, M. T
  7. J. Dent. Res v.74 no.5 Effects of unpolymerized resin components on the function of accessory cells derived from the rat incisor pulp Jontell, M., Hanks, C. T., Bratel, J., Bergenholtz, G
  8. Theses collection of Sookmyoung Women's University v.27 A study on the modulation of cell-mediated immunity by cyclophosphamide Pyo. M. Y
  9. Selected methods in cellular immunology Mishell, B;Shiigi, S
  10. J. Immunol v.14 Further improvements in the plaque technique for detecting single antibody-forming cells Cunningham, A;Szenberg, A
  11. Nature v.250 Decreased antibody formation in mice exposed to lead Loren, D. K
  12. Current protocols in immunology-National institute of health Coligan, J. E., Kruisbeek, A. M., Margulies, D. H., Shevach, E. M;Strober, W
  13. ELISA. J. Immunol v.109 no.1 Enzyme-linked immunosorbent assay Engvall, E;Perlmann, P
  14. Exp. Immunol v.10 Selective depletion of lymphoid tissue by cyclophosphamide Turk, T. L., Poulter, L. W
  15. Korean J. Toxicol v.7 Hematological values in normal laboratory mice(ICR and ddY strains) Guest, I. C., Paik, N. W., Ryu, J. C., Park, J. S;Chang, I. M
  16. Science v.153 Immunization of normal mouse spleen cell suspensions in vitro Robert, I;Richard, W
  17. Immunology Roitt, I., Brostoff, J., Male, D
  18. J. of Korean Cancer Association v.23 no.3 In Vitro cytotoxicity of various anticancer drugs to short-term cultured gastric adenocarcinoma cell lines Roh, J. K., Chung, H. C., Koh, E. H., Lee, W. Y., Hahn, J. S;Kim, B. S
  19. Cell & Tissue Culture; Laboratory procedures Dolye A., Griffiths J. B;Newell D. G