Environmental Analysis Health and Toxicology

The Korean Society of Environmental Health and Toxicology (환경독성보건학회)
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Studies on the Endocrine Disruption in Wildlife Fish

어류생체지표를 이용한 내분비계장애 연구

  • 구자민 (인천광역시 서구 경서동 환경연구단지내 국립환경연구원 환경위해성연구부) ;
  • 류지성 (인천광역시 서구 경서동 환경연구단지내 국립환경연구원 환경위해성연구부) ;
  • 정규혁 (성균관대학교 약학부) ;
  • 이철우 (인천광역시 서구 경서동 환경연구단지내 국립환경연구원 환경위해성연구부) ;
  • 박응로 (인천광역시 서구 경서동 환경연구단지내 국립환경연구원 환경위해성연구부) ;
  • 박광식 (인천광역시 서구 경서동 환경연구단지내 국립환경연구원 환경위해성연구부)
  • Published : 2001.12.01
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Abstract

Endocrine disruption in crucian carp (Carassius auratus) living in the branch of Han River were examined. Vitellogenin level in plasma was measured using ELISA system and aromatase mRNA level in brain was observed using RT-PCR technique. In all female fish, vitellogenin levels were in the range of 20∼40 $\mu\textrm{g}$/ml and aromatase mRNA expression could be detected on the agarose gel after RT-PCR. However, in case of males, vitellogenin level was elevated in only one fish, while vitellogenin was hardly detected in others. Aromatase was expressed in all males although the levels were relatively lower than the level in female fish. Testis-ova and any other histological changes of reproductive organ were not shown in both sexes.

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