Quantitative Determination of Eleutheroside B and I from Acanthopanax Species by High Performance Liquid Chromatography

  • 발행 : 2001.10.01

초록

Reversed-phase high performance liquid chromatographic method was applied for the determination of eleutheroside B and E in the various Acanthopanax species collected In Korea. The stationary phase used was Zorbax 300 SB $C_{18}$ and a mobile phase program was used, which started at 6% acetonitrile for 2 min, and then a linear gradient was operated for the next 18 min to 17% acetonitrile at a flow rate of 1.0 ml/min. The column effluent was monitored at UV 210 nm. Identification was carried out by comparing the retention time and the LC/MS spectrum of each peak corresponding to eleutheroside B and E from sample with those of standards. In general, the contents of eleutheroside B and 1 in stems were higher than those In roots. Acanthopanax species could be classified into two groups based upon the contents of eleutheroside B and E: one group contains no or very little eleutheroside B and another contains both eleutheroside B and E.

키워드

참고문헌

  1. Economic and Medicinal Plant Research Siberian Ginseng(Eleutherococcus senticosus):Current Status as an Adaptogen Farnsworth,N.R.;Kinghorn,A.D.;Sobe,S.;Protectivejarto,D.D.;Waller,D.P.;Wagner,H.(ed.);Hikino,H.(ed.);Farnsworth,N.R(ed.)
  2. Biol. Pharm. Bull. v.19 Nishie effects of Acanthopanax senticosus Harms from Hokkaido and its components on gastric ulcer inrestrained cold water stressed rats Fujikawa,T.;Yamaguchi,A.;Morita,I.;atoh,T.;Fujisa,Takeda,H.
  3. Oncol. Rep. v.7 Induction of apoptosis by Acanthopanax senticosus HARMS and its component,sesamin in human stomach cancer KATOⅢ cells Hibasami,H.;Fujikawa,T.;Takeda,H.;Nishibe,S.;Swa,T.;Nakashima,K.
  4. Ph.D.Dissertation,Seoul National University Studies on the chemical components and biological activities of Acanthopanax koreanum Kim,Y.H.
  5. J. Nat. Prod. v.51 Pimaradiene diterpenes from Acanthopanax koreanum Kim,Y.H.;Chung,B.S.;Sankawa,U.
  6. Exp. Hematol v.16 Radioprotection of hemopoiesis conferred by Acanthopanax senticosus Harms(Shigoka)administered before or after irradiation Miyanomae,T.;Frindel,E.
  7. Chem. Pharm. Bull. v.38 Phenolic compounds from stem bark of Acanthopanax senticosus and their pharmacological effect in chronic swimming stressed rats Nishibe,S.;Kinoshita,H.;Takeda,H.;Okano,G.
  8. J. Nat. Prod. v.63 New 3,4-seco-lupane-type triterpene glycosides from Acanthopanax senticosus forma inermis Park,S.Y.;Chang,S.Y.;Yook,C.S.;Nohara,T.
  9. Medicinal plants of East and Southeast of Asia Perry,L.M.;Metzger,J.
  10. Clin.Exp.Immunol v.118 Suppressive effect of Chinese medicinal herb,Acanthopanax gracilistylus,extract on human lymphocytes in vitro Shan,B.E.;Yoshita,Y.;Sugiura,T.;Yamashita,U.
  11. Int. J. Immunopharmacol v.13 Immunomopharmacological effects of polysaccharides from Acanthopanax senticosus on experimental animals Shen,M.L.;Zhai,S.K.;Chen,H.L.;Luo,Y.D.;Tu,G.R.;Ou,D.W.
  12. Shoyakugaku Zasshi v.39 Effect of Eleutherococcus senticosus and its rectal temperature,body and grip tones,mortor coordination,and exploratory and spontaneous movements in acute stressed mice Takasugi,N.;Moriguchi,T.;Fuwa,T.;Sanada,S.;Ida,Y.;Shoji,J.;Saito,H.
  13. Cancer Lett. v.65 Antitumor activity of polysaccharide from a Chinese medicinal herb, Acanthopanax giraldii Harms Wang,J.Z.;Tsumura,H.;Shimura,K.;Ito,H.
  14. Planta Med. v.57 Immunomodulatory activity of polysaccharide from Acanthopanax obovatus roots Wang,J.Z.;Mao,X.J.;Ito,H.;Shimura,K.
  15. Kor. J. Pharmacog. v.7 A new forma of Acanthopanax species(Ⅰ) Yook,C.S.;Lee,D.H.;Seo,Y.K.