Archives of Pharmacal Research
- Volume 24 Issue 2
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- Pages.144-149
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- 2001
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- 0253-6269(pISSN)
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- 1976-3786(eISSN)
Sphingolipid Metabolic Changes during Chiral C2-Ceramides Induced Apoptosis in Human Leukemia Cells
- Baek, Mi-Young (College of Pharmacy, Chungbuk National University) ;
- Yoo, Hwan-Soo (College of Pharmacy, Chungbuk National University) ;
- Kazuyasu Nakaya (Faculty of Pharmaceutical Science, Showa University) ;
- Moon, Dong-Cheul (College of Pharmacy, Chungbuk National University) ;
- Lee, Yong-Moon (College of Pharmacy, Chungbuk National University)
- Published : 2001.04.01
Abstract
N-acetylsphingosine (C2-ceramide) is a synthetic water-soluble ceramide mimicking the activity of natural ceramides. By fixing chiral conformation on carbon numbers 2 and 3 in the ceramide structure, four chiral C2-ceramides naming d-erythro-, I-erythro-, d-threo-and 1-three C2-ceramide were synthesized. We have investigated the chiral effects of these C2-ceramides on the sphingolipid metabolism, particularly on both the sphingolipid bio- synthetic pathway and on the degradation pathway. In both HL-60 and U937 cells, the chiral C2-ceramide (