한국가금학회지 (Korean Journal of Poultry Science)

  • 제28권2호
  • /
  • Pages.125-133
  • /
  • 2001
  • /
  • 1225-6625(pISSN)
  • /
  • 2287-5387(eISSN)
한국가금학회 (The Korean Society of Poultry Science)
권호 표지

수정란 배양 기술을 이용한 형질전환 닭 생산

Production of Transgenic Chicken by Using Embryo Culture Techniques

  • I. S. Jeon (National Livestock Research Institute, RDA) ;
  • D. H. Yoon (National Livestock Research Institute, RDA) ;
  • E. W. Park (National Livestock Research Institute, RDA) ;
  • W. G. Nho (National Livestock Research Institute, RDA) ;
  • C. H. Choi (National Livestock Research Institute, RDA) ;
  • J. H. Lee (National Livestock Research Institute, RDA) ;
  • H. H. Lee (National Livestock Research Institute, RDA) ;
  • I. C. Cheong (National Livestock Research Institute, RDA) ;
  • J. Y. Han (School of Agricultural Biotechnology, Seoul National University)
  • 발행 : 2001.08.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The goal of this paper was to examine the qualify zygote-acquiring method for in-vitro culture and the in-vitro culture method of the acquired zygote from a technological perspective. We have reported the results on the introduction of foreign DNAs using the described culturing method. After performing in-vitro and surrogate eggshell culture on a zygote acquired from the abdomen of a hen, 25.8% hatchability was acquired. After microinjecting foreign DNAs into the acquired zygote and performing in-vitro and surrogate eggshell culture using the same method, 13.1∼11.7% hatchability was acquired. Having compared the developments of the control subjects and the experimental subjects, the viability of the experimental subjects on the 4∼5th day of culturing was much lower compared to that of the control subjects. This is a result that shows that the microinjection process of foreign DNAs might have a negative effect on the existence of the embryo; therefore, various technical attempts should be made to minimize such negative effects. Having microinjected foreign DNAs into the zygote of a hen to produce transgenic chickens, 3 transgenic founders were Produced and 70 G1 progeny were produced as a result of the progeny test that had been performed to the present.

키워드

참고문헌

  1. Dev Biol v.41 A simple procedure for long term cultivation of chick embryos Auerbach,R.;Kubai,L.;Knighton,D.;Folkman,J.
  2. J Exp Biol v.61 Respiratory acidosis and eggshell resorption by the chick embryo Crooks,R.J.;Simkiss,K.
  3. Quarterly J Exp Physiol v.60 Calcium transport by the chick Chorioallantois in vivo Crooks,R.J.;Simkiss,K.
  4. Dev Biol v.88 Active calcium transport in the chick chorioallantoic membrnae required interaction with the shell membranse and/or shell calcium Dunn,B.E.;Graves,J.S.;Fitzharris,T.P.
  5. Poult Sci v.55 Growth of the chicken embryo in vitro Dunn,B.E.;Boone,M.A.
  6. Dev Biol v.71 Differentiation of the chorionic epithelium of chick embryos maintained in shell-less culture Dunn,B.E.;Fitzharris,T.P.
  7. BBA v.1192 The role of vitamin D in chorioallantoic membrance calcium transport Elaroussi,M.A.;Uhland-smith,A.;Hellwing,W.;DeLuca,H.F.
  8. Dev Biol v.49 From cleavage to primitive streak formation:a complementary normal table and a new look at the first stages of development of the chick Eyal-Giladi,H.;Kochav,S.
  9. Cell Defferentiation v.14 The gradual establishment of cell commitments during the early stage of chick development Eyal-Giladi H.
  10. Methods for the Introduction of Recombinant DNA into chicken Embryos. Transgenic animal First,N.;Haseltine,F.P.
  11. Korean J Anim Sci v.39 no.1 The surrogate egg-shell culture of quail embryos Jeon,I.S.;Choi,K.S.;Sohn,S.H.;Ono,T.
  12. the 8th World Conference on Animal Production Seoul Korea no.PB1-22 Production of transgenic chicken by DNA microinjection Jeon,I.S.;Mather,C.;Mitchell,R.;Glhooley,H.;Sherman,A.;Li,Y.;Sang,H.
  13. J Anim Sci & Technol(Kor.) v.42 no.6 Improvement of in vitro culture and surrogate egg-shell culture technique for single cell stage embryos of chicken Jeon,I.S.
  14. Dev Biol v.79 From cleavage to primitive streak formation: A complementary normal table and a new look at the first stage of the development Kochav,S.;Ginburg,M.;Eyal-Giladi,H.
  15. Bio/technol v.12 Trnasgenic birds by DNA microinjection Love,J.;Gribbin,C.;Mather,C.;Sang,H.
  16. Differentiation v.8 Cellular and biochemical parameters of growth of chick blastoderms during early morphogenesis McMaster,G.;Modak,S.P.
  17. J Exp Zool v.254 Development in culture of the chick embryo from fertilized ovum to hatching Naito,M.;Nirasawa,K.;Oishi,T.
  18. Brit Poult Sci v.32 Production of quail-chick chimeras by blastoderm cell tranfer Naito,M.;Watanabe,M.;Kinutani,M.;Nirasawa,K.;Oishi,T.
  19. Cell Technol v.10 no.7 Development in culture of the chick embryo from fertilized ovum to hatching Naito,M.
  20. Molecular Reproduction and Development v.39 Production of germline chimeric chickens, with high transmission rate of donor-derived gametes, produced by transfer of primordial germ cells. Naito,M.;Tajima,A.;Yasuda,Y.;Kuwana,T.
  21. J Exp Zool v.109 The site of fertilization in the domestic fowl Olsen,M.W.;Neher,B.H.
  22. Poult Sci v.62 Development of cultured quail embryos Ono,T.;Wakasugi,N.
  23. Poult Sci v.63 Mineral content of quail embryos cultured in mineral-rich and mineral-free condition Ono,T.;Wakasugi,N.
  24. Dev Biol v.161 A complete culture system for avian transgenesis, supporting quail embryos from the single cell stage to hatching Ono,T.;Murakami,T.;Mochii,M.;Agata,K.;Kino,K.;Otsuka,K.;Ohta,M.;Mizutani,M.;Yoshida,M.;Eguchi,G.
  25. Japanese Poult Sci v.31 Production of quail chimera by transfer of early blastodermal cells and its use for transfenesis Ono,T.;Muto,S.;Mizutani,M.;Agata,K.;Mochii,M.;Kino,K.;Otsuka,K.;Ohta,M.;Yoshida,M.;Eguchi,G.;
  26. Japanese Poult Sci v.32 no.4 Production of quail chimera by transfer of early blastodermal cells: Plumage chimeras and a germline chimera without plumage mixture Ono,T.;Muto,S.;Matsumoto,T.;Yoshida,M.
  27. Early embryology of the chick The process of Cleavage Patten,B.N.
  28. J.Morphol v.150 Nuclear events from fertilization to the early cleavage stages in the domestic fowl(Gallus domesticus) Perry,M.M.
  29. Nature v.331 A complete culture system for the chick embryo Perry,M.M.
  30. Avian incubation Satisfying the needs of the chick embryo in culture, with emphasis on the first week of development Perry,M.M.;Mather,C.M.
  31. Brit Poult Sci v.28 Explanted embryo culture:in vitro and in vivo technique for domestic fowl Rowlett,K.;Simkiss,K.
  32. Using transgenic technology for selection of traits in poultry. Compact disc. XXI World's Poultry Congress Sang,H.
  33. Nature Biotechnology v.16 Transposition of the Drosophila element mariner into chicken germ line Sherman,A.;Dawson,A.;Mather,C.;Gilhooley,H.;Li,Y.;Mitchell,R.;Finnegan,D.;Sang,H.
  34. Egg and eggshell quality The pattern of mineralisation Solomon,S.E.
  35. J Exp Zool v.147 Integrative mechanisms in the development of the early chick blastoderm Spratt,N.T.;Haas,H.
  36. Dev Biol v.74 Calcium transport and related functions in the chorioallantoic membrane of cultured shellless chick embryos Tuan,R.S.