Journal of Embryo Transfer (한국수정란이식학회지)
- Volume 16 Issue 3
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- Pages.223-231
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- 2001
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- 2508-755X(pISSN)
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- 2288-0178(eISSN)
Activation of Bovine Oocytes by Combined Treatment with Ionomycin and cdc2 Kinase Inhibitor
- Yoo, J.G. (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
- Cho, S.R. (Division of Applied Life Science, Gyeingsang National University) ;
- Lee, S.L. (Institute of Animal Medicine, College of Veterinary Medicine, Gyongsang National University) ;
- Ock, S.A. (Department of Biology, Gyeingsang National University) ;
- Rho, G.J. (Institute of Animal Medicine, College of Veterinary Medicine, Gyongsang National University) ;
- Son, D.S. (National Livestock Research Institute) ;
- Lee, H.J. (Institute of Animal Medicine, College of Veterinary Medicine, Gyongsang National University) ;
- Choe, S.Y. (Institute of Animal Medicine, College of Veterinary Medicine, Gyongsang National University)
- Published : 2001.12.01
Abstract
The success of nuclear transplantation with mammalian oocytes depends critically on the potential of oocytes activation, which mainly caused to prevent the re-accumulation of maturation promoting factor (MPF). This study was conducted to compare the effect of combined treatment of lonomycin with a Hl-histone kinase inhibitor (dimethylaminopurine, DMAP) or cdc2 kinase inhibitor (sodium pyrophosphate, SPP) on activation of bovine oocytes. In vitro matured bovine oocytes with the first polar body (PB) and dense cytoplasm were assigned to 3 experimental groups. For activation treatment, oocytcs were exposed to 5