Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Evaluation of the EF-18 Agar-Hydrophobic Grid Membrane Filter (HGMF) Method to Isolate Salmonella from Poultry Products

  • Rosa Capita (Department of Food Hygiene and Food Technology, Veterinary Faculty, University) ;
  • Astorga, Maite-Alvarez (Department of Food Hygiene and Food Technology, Veterinary Faculty, University) ;
  • Calleja, Carlos-Alonso (Department of Food Hygiene and Food Technology, Veterinary Faculty, University) ;
  • Maria del Camino (Department of Food Hygiene and Food Technology, Veterinary Faculty, University) ;
  • Garcia-Fernandez (Department of Food Hygiene and Food Technology, Veterinary Faculty, University) ;
  • Benito Moreno (Department of Food Hygiene and Food Technology, Veterinary Faculty, University)
  • Published : 2001.09.01
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Abstract

The EF-18 agar/hydrophobic grid membrane filter (EF18/HGMF) method was evaluated for the isolation of Salmonella in naturally contaminated chicken carcasses, chicken parts (legs, wings and giblets) and processed chicken products (sausages and hamburgers). Percentages of false positive results for Salmonella (colonies with a similar morphology to those of Salmonella) were 78.75, 81.67 and 80% for carcasses, chicken parts and processed chicken products, respectively. The bacterial isolates that caused false positive reactions using this method were identified as Proteus mirabilis (70.85%), Citrobacter freundii (15.25%), Klebsiella ozaenae (5.83%), Hafnia alvei (4.48%), Escherichia coli (2.69%) and Enterobacter aerogenes (0.90%). The data obtained in this study suggest that the EF-18/HGMF method is not sufficiently selective or specific far isolating Salmonella from meat and chicken products.

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