Cytokine-Inducing and T Cell Mitogenic Effects of Cordyceps hepialidicola

  • 발행 : 2001.09.01

초록

The morphological characteristics of newly isolated Cordyceps hepialidicola were characterized, and the phylogenetic relationships with other Cordyceps species were investigated using a sequence analysis of the internal transcribed spacer (ITS). The PCR product of 592 bp showed a homology of 92 and 91% with C. militaris and C. nutans, respectively, In an in vitro model using mouse peripheral blood mononuclear cells (PBMC), a methanol extract of C. hepialidicola induced multiple cytokines, including IFN-${\gamma}$ IL-4, and IL-18. The extract also enhanced the percentages of the CD4$\^$+/ and CD8$\sub$+/ T cells in the healthy murine PBMCs to 56.1% and 13.0%,respectively. The percentages of CD4$\^$+/ and CD8$\^$+/ in the untreated controls were 28.4 and 7.3%, and concanavalin A-treated positive controls were 62.4 and 18.3%, respectively.

키워드

참고문헌

  1. Immunol.Today v.17 IL-12 : a key cytokine in immune regulation Alan G.L.;A.Luciano
  2. Blood v.80 Human B-cell interleukin-10: B-cell lines derived from patients with acquired immunodeficiency syndrome and Burkitts lymphoma constitutively secrete large quantities of interleukin-10 Benjamin D.;T.J.Knobloch;M.A.Dayton
  3. Scand.J.Clin.Invest. v.21 Isolation of mononuclear cells and granulocytes from human blood Boyum A.
  4. J.Immunol. v.164 A prominent role for Sp1 during lipopolysaccharide mediated induction of the IL-10 promotor in macrophages Brightbill H.D;S.E.Plevy;R.L.Modlin;S.T.Smale
  5. Proc.Natl.Acad.Sci.USA v.88 Coexpression of two distinct genes is required to generate secreted bioactive cytotoxic lymphocyte maturation factor Gubler U.;A.O.Chua;D.S.Schoenhaut;C.M.Dwyer;W.McComas;R.Motyka;N.Nabavi;A.G.Wolitzky;P.M.Quinn;P.C.Familletti
  6. Ann.Rev.Immunol. v.19 Interleukin-10 and the interleukin-10 receptor Kevin W.M.;de W.M.Rene;L.C.Robert;O.G.Anne
  7. Cancer Invest. v.12 Growth inhibitors against tumor cells in Cordyceps sinensis other than cordycepin and polysaccharides Kuo Y.C;C.Y.Lin;W.J.Tsai;C.L.Wu;C.F.Chen;M.S.Shiao
  8. Curr.Opin.Immunol. v.10 Regulation of interferon-g production by IL-12 and IL-18 Okamura H.;S.Kashiwamura;H.Tsutsui;T.Yoshimoto;K.Nakanishi
  9. J.Microbiol.Biotechnol. v.10 Antitumor activity of Paecilomyces japonica is mediated by apoptotic cell death Park Y.H.;E.K.Moon;Y.K.Shin;M.A.Bae;J.G.Kim;Y.H.Kim
  10. Gene Expression v.5 The pathway to maturity: Processing of ribosomal RNA in Saccharomyces cerevsiae Raue H.A.;R.J.Planta
  11. Kor.J.Mycol. v.23 Classification of Cordyceps spp.by morphological characteristics and protein banding pattern Sung J.M.;H.K.Lee;K.Y.Yang
  12. Mycoscience v.39 Phylogenetic analysis and predicted secondary structures of the rDNA internal transcribed spacers of the powdery mildew fungi (Erysiphaceae) Takamatsu S.;T.Hirata;Y.Sato
  13. J.Immunol. v.165 IL-10 gene expression is controlled by the transcription factors Sp1 and Sp3 Tone M.;M.J.Powell;Y.Tone;S.A.Thompson;H.Waldmann
  14. Ann.Rev.Immunol. v.13 Interleukin-12: A proinflammatory cytokine with immunoregulatory functions that bridge innate resistance and antigen-specific adaptive immunity Trichieri G.
  15. Amplification and direct sequencing of fungal ribosomal genes from phylogenetics White T.J.;T.D.Bruns;S.Lee;J.Taylor;M.A.Innis(ed.);D.H.Gelfrand(ed.);J.J.Sinsky(ed.);T.J.White(ed.)
  16. J.Immunol. v.146 Cloning of cDNA for natural killer cell stimulatory factor,a heterodimeric cytokine with multiple biologic effects on T and natural killer cells Wolf S.F.;P.A.Temple;M.Kobayashi;D.Young;M.Dicig;L.Lowe;R.Dzialo;L.Fitz;C.Ferenz;R.M.Hewick