Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
권호 표지

Improvement in the Stability of Glycinecin A through Protein Fusion of the Two Structural Components

  • Kim, Youngmee (Department of Biochemistry, Cheju National University Medical School) ;
  • Somi K. Cho (Department of Biochemistry, Cheju National University Medical School) ;
  • Moonjae Cho (Department of Biochemistry, Cheju National University Medical School)
  • Published : 2001.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

Glycinecin A, a bacteriocin produced by Xanthomonas campestris pv. glycines, inhibits the growth of X. c. pv. vesicatoria. We have reported that purified glycinecin A is composed of two polypeptides, is active over a wide range of pH (6 to 9), and is stable at temperatures up to 60$\^{C}$. Glycinecin A is a heterodimer consisting of 39- and 14-kDa subunits; the two encoding genes, glyA and glyB, respectively, have been cloned (Heu et al. 2001. Appl. Environ. Microbiol. 67, 4105-4110). Co-expression of glyA and glyB in the same cell is essential for bacteriocin activity. We constructed and produced a chimeric glycinecin A connecting glyA and glyB in one open reading frame. The chimeric glycinecin A has the same bactericidal activity as the wild-type glycinecin A. However, the chimeric glycinecin A is more stable in a wider range of pH and temperature.

Keywords

References

  1. J.Gen Microbiol. v.131 Purification and characterization of a Corynebacterium ulcerans bacteriocin (ulceracin 378) Abrehem K.;I.Zamiri
  2. J.Bacteriol. v.176 Expansion of bacteriocin activity and host range upon complementation of two peptides encoded by the lactacin F operon Allison G.;C.Fremaux;C.Ahn;T.R.Klaenhammer
  3. J.Bacteriol. v.181 Identification and cloning of an Erwinia carotovora subsp.carotovora bacteriocin regulator gene by insertional mutagenesis Chuang D.Y.;A.G.Kyeremeh;Y.Gunji;Y.Takahara;Y.Ehara;T.Kikumoto
  4. Curr.Microbiol. v.16 Bacteriocins and temperate phage of Xanthomonas campestris pv.glycines Fett W.F.;M.F.Dunn;G.T.Maher;B.E.Maleeff
  5. Appl.Environ Microbiol. v.67 Cloning,expression,and purification of glycinecin A,a bacteriocin produced by Xanthomonas campestris pv.glycines 8ra Heu S.;Oh J.H;Kang Y.S;Ryu S.;Cho S.K;Cho Y.S;M.Cho.
  6. Bacteriocins,Microcins and Lantibiotics James R.;C.Lazdunski;F.Pattus(ed.)
  7. Plant Path.J. Effect of Glycinecin A on the Control of Bacterial Leaf Spot of Red Pepper and Bacterial Leaf Blight of Rice Jeon Y.H.;Cho M.;Cho Y.S.;Hwang I.
  8. Annu.Rev.Microbiol. v.46 Genetics of ribosomally synthesized peptide antibiotics Kolter R.;F.Moreno
  9. J.Biol.Chem. v.241 The release of enzymes by osmotic shock from Escherichia coli in exponential phase Nossal N.G;L.A.Heppel
  10. Microbiology v.145 Synthesis of lactococcin 972,a bacteriocin produced by Lactococcus lactis IPLA 972,depends on the expression of a plasmid-encoded bicistronic operon Martinez B.;M.Fernandez;J.E.Suarez;A.Rodriguez
  11. Methods in Microbiology v.7A Methods for studying bacteriocins Mary-Harting A.;A.J.Hedges;R.C.W.Berkely;J.R.Norris(ed.);D.W.Ribbons(ed.)
  12. J.Bacteriol. v.174 A novel lactococcal bacteriocin whose activity depends on the complementary action of two peptides Nissen-Meyer J.;H.Holo;L.S.Havarstein;K.Sletten;I.F.Nes
  13. Microbiol. v.142 Structural and functional organization of the Yersinia pestis bacteriocin pesticin gene cluster Rakin A.;E.Boolgokowa;J.Heesemann
  14. Mol.Biol.Evol. v.10 Molecular mechanisms of colicin evolution R.A.Riley
  15. Nature v.265 Agrocin 84 is a 6-N -phosphoramidate of an adenine nucleotide analogue Roberts W.P;M.E.Tate;A.Kerr
  16. App.Environ.Microbiol. v.61 Improvement of solubility and stability of the Anti-microbial peptide nisin by protein engineering Rollema H.S;Kuipers O.P;Both P;DeVos W.M;R.J.Siezen
  17. Mol.Gen.Genet v.237 A novel transposon-like structure carries the genes for pyocin AP41,a Pseudomonas aeruginosa bacteriocin with a DNase homology to E2 group colicins Sano Y;M.Kageyama
  18. Microbiology and biogeochemistry of hypersaline environments Halocins: protein antibiotics from hypersaline environments Shand R.D;L.B.Price;E.O'Connor;A.Oren(ed.)
  19. Can.J.Microbiol. v.32 Isolation and characterization of syingacin W-1,a bacteriocin produced by Pseudomonas syringae pv.syringae Smidt M.L;A.K.Vidaver
  20. J.Gen.Microbiol. v.135 Halocin H6,a bacteriocin from Haloferax gibbonsii Torreblanca M;I.Meseguer;F.Rodriguez-Valera
  21. Bacteriocins of Lactic Acid Bacteria Vuyst L.D;E.Vandamme(ed.)
  22. Phytopathol. v.81 Plasmid,genetic,and bacteriocin diversity in U.S.strains of Xanthomonas campestris pv.oryzae Xu G.;C.F.Gonzalez