Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Antibacterial Activities of Lactobacillus crispatus ATCC 33820 and Lactobacillus gassed ATCC 33323

  • Kim, Jin-Woo (Department of Chemical and Biochemical Engineering, University) ;
  • S.N. Rajagopal (Department of Microbiology, University of Wisconsin-La Crosse)
  • Published : 2001.06.01
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Abstract

Lactobacillus crispatus ATCC 33820 and L. gasseri ATCC 33323 were grown in MRS broth (pH 6.5) at 37$^{\circ}C$ for 24 h and the antibacterial activities of cell free culture supernatants were determined by the agar well diffusion method. The culture supernatants were inhibitory to Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Bacillus subtilis, Staphylococcus aureus, Enterococcus faecalis, Pediococcus acidilacticii, and Lactobacillus helveticus. The supernatants did not show any lysozyme activity. Addition of catalase did not affect the antibacterial activities of the supernatants. The antibacterial substances were heat stable (100$^{\circ}C$ for 60 min) and sensitive to proteases.

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