Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
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Differential Expression of Neuropetide Y in the Hypothalamic Areas of Fasting and Anorexia Mutant Mice

Neuropeptide Y에 의한 식욕조절 관찰연구

  • 김미자 (동덕여자대학교 자연과학대학 식품영양학과) ;
  • 김영옥 (동덕여자대학교 자연과학대학 식품영양학과) ;
  • 김혜경 (한서대학교 식품생물공학과) ;
  • 정주호 (경희대학교 의과대학 약리학교실)
  • Published : 2001.10.01
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Abstract

The present study was conducted to identify the mechanism about the regulation of appetite by examining the expression patterns of neuropeptide Y in the hypothalamus of mice either fasting mouse for 24 hours or with anorexia mutant mouse. In order to investigate the patterns of expression of neurpeptide Y, immunohistochemistry was employed for measurements at the tissue level, along with the molecular biological techniques of reverse transcription polymerase chain reaction(RT-PCR) and dot blotting. The results of this study are as follows. The level of expression of neruopeptide Y, a neuropeptide known to enhance appetite, was shown to be lowered in the arcuate nucleus(ARC), paraventricular nucleus(PVN), lateral hypothalamic area(LHA), and dorsomedial hypothalamic nucleus(DMN) in both the fasting and anorexia mutant groups when measured via immunohistochemistry, a tissue-level method. RT-PCR and dot blotting, the molecular biological methods employed in this study, revealed that the level of neuropeptide Y mRNA in the entire hypothalamus was similar in the control and fasting groups and lower in the anorexia mutant group. The results of the present study showed that while the levels of expression of the neuropeptide Y in the various hypothalamic regions studied did not exhibit regular increases or decreases when measured immunohistochemically. But the entire hypothalamus via molecular biological methods showed that the changes in these levels were more definite in the anorexia mutant group than in the fasting group.

Keywords

References

  1. Nature v.404 Central nervous system control of food intake Schwatz MW;Woods SC;Porte Jr D;Seeley RJ;Baskin DG
  2. Physiol Rev v.79 Regulation of body weight in humans Jquier E;Tappy L
  3. Fed Proc v.45 Brain monoamines and peptides : role in the control of eating behavior Leibowitz SF
  4. Proc Natl Acad Sci USA v.95 The hypocretins : hypothalamus-specific peptides with neuroexcitatory activity de Lecea L;Kilduff TS;Peyron C;Gao X;Foye PE;Danielson PE;Fukuhara C;Battenberg EL;Gautvik VT;Bartlett FS Jr;Frankel WN;van den Pol AN;Bloom FE;Gauvik KM;Sutcliffe JG
  5. Proc Natl Acad Sci USA v.88 Neuropeptide Y secretion increases in the paraventricular nucleus in association with increased appetite for food Kalra SP;Dube MG;Sahu A;Phelps CP;Kalra PS
  6. Neuron v.22 From lesions to leptin : hypothalamic control of food inkake and body weight Elmquist J;Elias C;Saper C
  7. Endocr Rev v.20 Interacting appetite-regulating pathways in the hypothalamic regulation of body weight Kalra SP;Dube MG;Pu S;Xu B;Horvath TL;Kalra PS
  8. Science v.221 Neuropeptide Y distribution in the rat brain Allen YS;Adrian TE;Allen JM;Tatemoto K;Crow TJ;Bloom SR;Polak JM
  9. Peptides v.9 Food deprivation and ingestion induce reciprocal changes in neuropeptide Y concentrations in the paraventricular nucleus Sahu A;Kalra PS;Kalra SP
  10. Brain Res Mol Brain Res v.11 Food deprivation and hypothalamic neuropeptide gene expression : effects of strain background and the diabetes mutant Chua SC Jr
  11. Peptides v.6 Neuropeptide Y and Peptide YY neuronal and endocrine systems O'Donohue TL;Chronwall BM;Pruss RM;Mezey E;Kiss JZ;Eiden LE;Massari VJ;Tessel RE;Pickel VM;DiMaggio DA
  12. DiAm J Physiol v.266 Neuropeptide Y in hypothalamic paraventricular nucleus : a center coordinating energy metabolism Billington CJ;Briggs JE;Harker S;Gace M;Levine AS
  13. Diabet Med v.6 Elevated neuropeptide Y concentrations in the central hypothalamus of the spontaneously diabetic BB/E Wistar rat Williams G;Lee YC;Chatei MA;Cardoso HM;Ball JA;Bone AJ;Baird JD;Bloom SR
  14. Endocrinology v.132 Increased neuropeptide-Y messenger ribonucleic acid(mRNA) and decreased neurotensin mRNA in the hypothalamus of the obse(ob/ob) mouse Wilding JP;Gilbey SG;Bailey Cj;Batt RA;Williams G;Ghatei MA;Bloom SR
  15. J Hered v.75 Anorexia, a recessive mutant causing starvation in preweanling mice Maltais LJ;Lane PW;Beamer WG
  16. Korean J Nutrition v.33 Expression of tryptophan hydroxylase in the hypothalamus and hippocampus of fasting and anorexia mutant mice Kim MJ;Kim YO;Chung JH
  17. Korean J Nutrition v.33 Expression of Neuropeptide Y(neuropeptide Y) and NADPH-diaphorase Neurons in the hypothalamus and cerebral cortex of fasting and anorexia mutant mice(anx/anx) Kim YO;Kim MJ;Chung JH
  18. The Mouse Brain in Steretaxic Coordinates Franklin KBJ;Paxinos G
  19. J Comp Neurol v.387 Hypothalamic neurohistochemistry of the murine anorexia(anx/anx) mutation : altered processing of neuropeptide Y in the arcuate nucleus Broberger C;Johansen J;Schalling M;Hkfelt T
  20. Brain Res v.790 Neuropeptide Y mRNA and serotonin innervation in the arcuate mucleus of anorexia mutant mice Jahng JW;Houpt TA;Kim SJ;Joh TH;Son JH
  21. Diabetes v.48 Leptin receprot mRNA identifies in rat hypothalamus Baskin DG;Breininger JF;Schwartz MW
  22. Comp Biochem Physiol v.106 Refeeding after 72 hour fasting alters neuropeptide Y and monoamines in various cerebral areas in the rat Pages N;Orosco M;Rouch C;Yao O;Jacquot C;Bohuon C
  23. Pharmacol Biochem Behav v.44 Fasting affects more markedly neuropeptide Y than monoamines in the rat brain Pages N;Orosco M;Rouch C;Yao O;Jacquot C;Bohuon C
  24. Am J Clin Nutr v.50 Genetic and hypothalamic mechanims for obesity-finding the needle in the haystack Bray GA
  25. Neuroscience v.72 The serotonergic agent fluoxetine reduces neuropeptide Y levels and neuropeptide Y secretion in the hypothalamus of lean and obse rats Dryden S;Frankish HM;Wang Q;Pickavance L;Williams G
  26. Nat Med v.4 Role of the Y5 neuropeptide Y receptor in feeding and obesity Marsh DJ;Hollopeter G;Kafer KE;Palmiter RD
  27. Proc Natl Acad Sci USA v.95 Obesity and mild hyperinsulinemia found in neuropeptide Y-Y1 receptor-deficient mice Kushi A;Sasai H;Koizumi H;Takeda N;Yokoyama M;Nakamura M