Supercritical Fluid Extraction of Catecnin Compounds from Green Tea

녹차에서 카테킨 화합물의 초임계 유제 추출

  • 나영진 (초정밀분리기술센터, 인하대학교 화학공학과, 국가지정 초임계 유체 연구실) ;
  • 이윤우 (한국과학기술연구원) ;
  • 김재덕 (한국과학기술연구원) ;
  • 노경호 (초정밀분리기술센터, 인하대학교 화학공학과, 국가지정 초임계 유체 연구실)
  • Published : 2001.08.01

Abstract

Catechin compounds from the Green tea cultivated in Bosung (Chollanamdo) were extracted using supercritical fluid and various additives, and analyzed by reversed-phase high performance liquid chromatography (RP-HPLC). The chromatographic column was packed with LiChrospher 100RP-18(15 $\mu$m), and water was used as the mobile phase with 0.05% phosphoric acid and acetonitrile. Gradient election was applied to separate EGCG by changing the mobile phase compositions. Comparing the extraction yield of three different types of supercritical fluids, pure CO$_2$, with additives of water and ethanol (5 wt,%), the extraction amount of EGCG was most abundant in the suprcritical CO$_2$with ethanol. However, more was extracted and pure higher purity was achieved by solvent extraction using ethanol.

전라남도 보성산 녹차로부터 유용성분인 카테킨 화합물을 초임계 이산화 탄소와 첨가제를 사용하여 추출하고 역상 고성능 액체 크로마토그래피(RP-HPLC)를 사용하여 분석하였다. LiChrospher 100RP-18(15 $\mu$m)로 충진된 column을 이용하여 0.05% phosphoric acid가 포함된 물과 acetonitrile을 이동상으로 사용하였으며 구배용매 조성법을 적용하였다. 이동상의 조성에 따른 체류인자의 조절을 통해 EGCG를 분리하였다. 각각의 초임계 추출법에 따른 EGCG의 추출량을 비교한 결과, 초임계 유체에 극성물질을 포함하는 경우가 녹차의 유용 성분인 EGCG의 추출량이 가장 많았다. 그러나 ethanol에 의한 용매 추출법은 초임계 유체에 의한 추출법보다 EGCG에 대해 많은 추출양과 선택적인 고순도 분리가 가능하였다.

Keywords

References

  1. The Journal of Supercritical Fluids v.19 Supercritical fluid extraction of wood pulp with analysis by capillary gas chromatography-mass spec-trometry McDaniel,L.H.;M.Achraf-Khorassani;L.T.Taylor
  2. Journal of Hazardous Materials v.69 no.2 Supercritical fluid extraction/~radiolysis of PCBs from contaminated soil Yak,H.K.;B.J.Mincher;K.H.Chiu;C.M.Wai
  3. J.Supercritical Fluids v.17 no.2 Solubilities of soild mixtures in supercritical carbon dioxide:a review Lucien,F.P;N.R.Foster
  4. Prev.Med. v.21 Oguni,I.;S.J.Chen;P.Z.Lin;Y.Hara
  5. Anti-Cancer Drugs v.7 Valcic,S.;B.N.Timmermann;D.S.Alberts;G.A.Wchter;M.Krutzsch;J.Wymer;J.M.Guillen
  6. Theories and Applications of Chemical Engineering v.6 Hong,S.B.;J.I.Kim;K.H.Row
  7. Wat.Res. v.30 no.2 David G.;P.A.Breen;J.M.Horner;K.D.Bartle;A.A.Clifford;James Waters;Graham Lawrence
  8. pending to Korean patent Chung,S.T.;K.H.Row;J.H.Kang;Y.K.Park
  9. Principles and Applications of Liquid Chromatography Row,K.H.
  10. J.Food Eng v.40 Solubility study of green tea extracts in pure solvents and edible oils Victor N.;M.Nakajima;J.Tong;S.Ichikawa
  11. Korean J.Chem.Eng. Separation of Perillyl Alcohol in Korean Orange Peel by Supercritical CO₂and Preparative High-Performance Liquid Chromatography Lee,C.H.;Y.W.Lee;J.D.Kim;Y.Y.Lee;K.H.Row