Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Enhancement of Xylitol Production Yield by Xylitol Dehydrogenase Defective Mutant of Pichia stipitis

Pichia stipitis의 Xylitol Dehydrogenase Defective Mutant에 의한 Xylitol 생산 수율 향상

  • Kim, Min-Soo (Department of Molecular Science and Technology, Division of Chemical Engineering and Biotechnology, College of Engineering, Ajou University) ;
  • Kim, Chul (Department of Molecular Science and Technology, Division of Chemical Engineering and Biotechnology, College of Engineering, Ajou University) ;
  • Seo, Jin-Ho (Department of Food Science and Technology, Seoul National University) ;
  • Ryu, Yeon-Woo (Department of Molecular Science and Technology, Division of Chemical Engineering and Biotechnology, College of Engineering, Ajou University)
  • 김민수 (아주대학교 화학생물공학부 및 분자과학기술학과) ;
  • 김철 (아주대학교 화학생물공학부 및 분자과학기술학과) ;
  • 서진호 (서울대학교 식품공학과) ;
  • 유연우 (아주대학교 화학생물공학부 및 분자과학기술학과)
  • Published : 2001.06.01
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Abstract

In order to produce xylitol with high yield, experiments were carried out to develope xylitol dehydrogenase(XDH) defective mutant from P. stipitis and to investigate the xylitol fermentation characteristics of mutant strain. After treatment of P. stipitis with EMS, mutant PXM-4 was selected based on te XDH activity and xylitol production capability. Among the tested cosubstrates, galactose was selected as an adequate cosub-strate on xylitol production of mutant PXM-4. But with the increase in the concentration of galactose in the medium, xylitol production was decreased because the transport of xylose into cell was inhibited by galactose. The optimal concentration of galactose for the production of xylitol using 20 g/ι xylose was 20 g/ι Under this condition, maximum concentration of xylitol and yield were 14.4 g/ι and 97%, respectively. In order to prevent the inhibitory effect of xylose transport by galactose, galactose was fed with low concentration and the concentration of xylitol produced was increased up to 25 g/ι.

본 연구에서는 높은 수율로 xylitol을 생산하기 위하여 P. stipitis CBS 5776으로부터 xylitol dehydrogenase(XDH)의 활성이 결여된 변이균주의 개발과 xylitol 발효특성에 관한 실험을 수행하였다. EMS(ethylmethane sulfonate)를 처리하여 XDH defective 변이균주인 PXM-4를 최종적으로 선별하였고, 변이균주 PXM-4의 XDH 활성을 측정함으로써 XDH 활서이 완전히 제거된 변이균주임을 확인하였다. 변이균주 PXM-4의 xylitol 발효에서 가장 적합한 cosubstrate로서 galactose를 선정하였다. Galactose와 xylose의 혼합당 배지에서 xylitol 발효를 수행한 결과 galactose의 농도가 20g/ι 이상에서는 xylitol 생산이 오히려 낮아졌고, 20g/ι의 xylose를 이용한 xylitol 발효에서 가장 적합한 galactose의 농도는 20g/ι이었으며, 생산된 xylitol의 농도는 14.4 g/ι이었고, 수율은 97%이었다. 또한 잔존하는 xylose로 완전히 xylitol로 전환시키기 위해 xylitol 농도가 증가되지 않는 시기에 galactose를 첨가함으로써 최종 xylitol의 농도는 25g/ι로 향상되었다. 이와 같은 결과에 따라 XDH defective 변이균주의 개발과 배양 조건을 최적화함으로써 높은 수율의 xylitol 생산이 가능함을 확인하였다.

Keywords

References

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