Journal of Microbiology and Biotechnology

  • 제11권5호
  • /
  • Pages.895-898
  • /
  • 2001
  • /
  • 1017-7825(pISSN)
  • /
  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Molecular Cloning and Characterization of the doxA Cytochrome P-450 Hydroxylase Gene in Streptomyces peucetius subsp. caesius ATCC 27952

  • Hong, Young-Soo (Anticancer Research Laboratory, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Hang-Sub (Anticancer Research Laboratory, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Jeong-Hyung (Anticancer Research Laboratory, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Kyu-Won (Department of Molecular Biology, Pusan National University) ;
  • Lee, Jung-Joon (Anticancer Research Laboratory, Korea Research Institute of Bioscience and Biotechnology)
  • 발행 : 2001.10.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

DNA sequence analysis of doxA from Streptomyces peucetius subsp. caesius ATCC 27952 revealed a $95\%$ amino acid identity with that of Streptomyces strain C5. DoxA from S. peucetius subsp. caesius ATCC 27952 encodes a peptide with both conserved heme-binding and dioxygen-binding motifs. Expression of this gene in S. lividans 1326 resulted in bioconversion of daunorubicin to doxorubicin.

키워드

참고문헌

  1. Med. Res. Rev. v.4 Antitumor anthracycline: Recent developments Arcamone, F.
  2. Biotechnol. Bioeng. v.11 Adriamycin, a new antitumor antibiotic from Streptomyces peucetius var. caeisu. Arcamone, F.;G. Cassinelli;G. Fantini;A. Grein;P. Orezzi;C. Poli;C. Spallia
  3. J. Bacteriol. v.178 Isolation and characterization of a gene from Streptomyces sp. strain C5 that confers the ability to convert daunomycin to doxorubicin on Streptomyces lividans TK24 Dickens, M. L.;W. R. Strohl
  4. Adv. Appl. Microbiol. v.32 Antitumor anthracyclines produced by Streptomyces peucetius Grein, A.
  5. Genetic Manipulation of Streptomyces. A Laboratory Manual Hopwood, D. W.;M. J. Bibb;K. F. Chater;T. Kieser;C. J. Bruton;H. M. Kieser;D. J. Lydiate;C. P. Smith;J. M. Ward;H. Schremph
  6. J. Microbiol. Biotechnol. v.7 Characterization of doxorubicin-nonproducing mutant, Nu23 of Streptomyces peuetius ATCC 27952 Hwang, C. K.;H. S. Lee;Y.-S. Hong;N. S. Park;T. H. Kim;J. J. Lee
  7. FEMS Microbiol. Lett. v.174 FramePlot: A new implementation of the frame analysis for predicting protein-coding regions in bacterial DNA with a high G + C content Ishikawa, J.;K. Hotta
  8. J. Bacteriol. v.181 Doxorubicin overproduction in Streptomyces peucetius: Cloning and characterization of the dnrU ketoreductase and dnrV genes and the doxA cytochrome P-450 hydroxylase gene Lomovskaya, N.;S. L. Otten;Y. Doi-Katayama;L. Fonstein;X. C. Liu;T. Takatsu;A. Inventi-Solari;S. Filippini;F. Torti;A. L. Colombo;C. R. Hutchinson
  9. Mol. Microbiol. v.20 Bacterial cytochrome P-450 Munro, A. W.;J. G. Lindsay
  10. Gene v.61 A new shuttle cosmid vector, pKC505, for streptomycetes: Its use in the cloning of three different spiramycin-resistance genes from a Streptomyces ambof aciens library Richardson, M. A.;S. Kuhstoss;P. Solenberg;N. A. Schaus;R. N. Rao
  11. Molecular Cloning: A Laboratory Manual(2nd Edition) Sambrook, J.;E. F. Fritsch;T. Maniatis
  12. J. Bacteriol. v.171 Cloning of genes governing the deoxysugar portion of the erythromycin biosynthesis pathway in Saccharopolyspora erythraea Vara, J.;M. Lewandowska-Sharbek;Y.-G. Wang.;S. Donadio;C. R. Hutchinson
  13. J. Bacteriol. v.181 Purification, properties, and characterization of recombinant Streptomyces sp. strain C5 DoxA, a cytochrome P-450 catalyzing multiple steps in doxorubicin biosynthesis Walczak, R. J.;M. L. Dickens;N. D. Priestley;W. R. Strohl
  14. J. Bacteriol. v.181 Purification, properties, and characterization of recombinant Streptomyces sp. strain C5 DoxA, a cytochrome P-450 catalyzing multiple steps in doxorubicin biosynthesis Walczak,R.J.;M.L.Dickens;N.D.Priestley;W.R.Strohl