Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Molecular Cloning and Characterization of the doxA Cytochrome P-450 Hydroxylase Gene in Streptomyces peucetius subsp. caesius ATCC 27952

  • Hong, Young-Soo (Anticancer Research Laboratory, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Hang-Sub (Anticancer Research Laboratory, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Jeong-Hyung (Anticancer Research Laboratory, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Kyu-Won (Department of Molecular Biology, Pusan National University) ;
  • Lee, Jung-Joon (Anticancer Research Laboratory, Korea Research Institute of Bioscience and Biotechnology)
  • Published : 2001.10.01
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Abstract

DNA sequence analysis of doxA from Streptomyces peucetius subsp. caesius ATCC 27952 revealed a $95\%$ amino acid identity with that of Streptomyces strain C5. DoxA from S. peucetius subsp. caesius ATCC 27952 encodes a peptide with both conserved heme-binding and dioxygen-binding motifs. Expression of this gene in S. lividans 1326 resulted in bioconversion of daunorubicin to doxorubicin.

Keywords

References

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