Effect of ArsA, Arsenite-Specific ATPase, on Inhibition of Cell Division in Escherichia coli

  • Lee, Sung-Jae (Research Institute for Basic Science and Department of Biology, Kyung Hee University) ;
  • Lee, Soo-Chan (Research Institute for Basic Science and Department of Biology, Kyung Hee University) ;
  • Choi, Seung-Ho (Research Institute for Basic Science and Department of Biology, Kyung Hee University) ;
  • Chung, Mi-Kyung (Research Institute for Basic Science and Department of Biology, Kyung Hee University) ;
  • Rhie, Ho-Gun (Research Institute for Basic Science and Department of Biology, Kyung Hee University) ;
  • Lee, Ho-Sa (Research Institute for Basic Science and Department of Biology, Kyung Hee University)
  • Published : 2001.10.01

Abstract

Escherichia coli, which harbored the ars operon from a plasmid pMH12 of Klebsiella oxytoca D12, showed filamentation due to the expression of ars genes in the presence of arsenite. The continued DNA replication in the absence of cell division was revealed, since nucleoids abound with DAPI appeared to be arranged in chains. In contrast to overexpression of arsA, its frame-shift mutant and knock-out mutant lost filamentation in the presence of arsenite, which suggested that ars-induced division block was dependent on expression of arsA. ArsA-induced division inhibition was not a consequence of an inhibition of DNA replication, and the inability of arsenite to induce an SOS response indicated that arsA-mediated division inhibition was dependent on the expression of the gene product encoded by the minB operon. ArsA is a peripheral membrane protein with an ATP-binding domain, which is homologous to MinD that requires ATP-dependent efflux. These results suggested that ArsA could possibly recruit MinC to the membrane and modulate cytoplasmic FtsZ to block assembly at the middle of the cell.

Keywords

References

  1. Bacterial Cell Wall Molecular biology of bacterial septation Ayala, J. A.;T. Garrio;M. A. De Pedro;M. Vicente;J. M. Ghuysen(ed.);R. Hakenbeck(ed.)
  2. Nature v.354 FtsZ ring structure associated with division in Escherichia coli Bi, E.;J. Lutkenhaus
  3. J. Bacteriol. v.175 Cell division inhibitors SulA and MinCD prevent formation of the FtsZ ring Bi, E.;J. Lutkenhaus
  4. FEMS Microbiol. Lett. v.139 The arsenical resistance operon of IncN plasmid R46 Bruhn, D. F.;J. Li;S. Silver;F. Roberto;B. P. Rosen
  5. Kor. J. Microbiol. v.31 Construction of a cloning vector carrying arsenic compound resistant genes Chung, M. K.;N. J. Chung;H. S. Lee
  6. J. Bacteriol. v.161 separate resistance to arsenate and arsenite (antimonate) encoded by the arsenical resistance operon of R factor R773 Chen, C.;H. L. T. Mobley;B. P. Rosen
  7. Cell v.56 A division inhibitor and a topological specificity factor coded for by the minicell locus determine proper placement of the division septum in E. coli de Boer, P. A. J.;R. E. Crossley;L. I. Rothfield
  8. Proc. Natl. Acad. Sci. USA v.87 Central role for the Escherichia coli minC gene product in two different cell division-inhibition systems de Boer, P. A. J.;R. E. Crossley;L. I. Rothfield
  9. EMBO J. v.10 The MinD protein is a membrane ATPase required for the correct placement of the Escherichia coli division site de Boer, P. A. J.;R. E. Crossley;A. R. Hand;L. I. Rothfield
  10. J. Bacteriol. v.177 Dual mode of energy coupling by the oxyanion-translocating ArsB protein Dey, S.;B. P. Rosen
  11. J. Bacteriol. v.171 Chromosome partitioning in E. coli: Novel mutants producing anucleate cells Hiraga, S. H.;T. Niki;C. Ogura;M. Ichinose;E. B. Mori;A. Jaffe
  12. J. Biol. Chem. v.264 Characterization of the catalytic subunit of an anion pump Hsu, C. M.;B. P. Rosen
  13. J. Microbiol. Biotechnol. v.11 Cloning and sequencing of the β-amylase gene from Paenibacillus sp. and its expression in Saccharomyces cerevisiae Jeong, T. H.;H. O. Kim;J. N. Park;H. J. Lee;D. J. Shin;H. B. Lee;S. B. Chun;S. Bai
  14. Mol. Microbiol. v.37 Cell division inhibitors SulA and MinC/MinD block septum formation at different steps in the assembly of the Escherichia coli division machinery Justice, S. S.;J. Garcia-Lara;L. I. Rothfield
  15. Ph. D. thesis. Kyung Hee University Studies on sequencing and characterization of arsenic salts resistance operon from Klebsiella oxytoca D12 plasmid pMH12 and expression of its genes in Escherichia coli Lee, S. J.
  16. Escherichia coli and Salmonella: Cellular and Molecular biology Cell division Lutkenhaus, J.;A. Mukherjee;F. C. Neidhardt(et al.)(ed.)
  17. Experiments in Molecular Genetics Miller, J. H.
  18. Mol. Gen. Genet. v.191 Cloning and expression of R-factor mediated arsenate resistance in Escherichia coli Mobley, H. L. T.;C. Chen;S. Silver;B. P. Rosen
  19. J. Microbiol. Biotechnol. v.10 Hormonal effects of several chemicals in recombinant yeast, MCF-7 cells and uterotrophic assay in mice Park, J. S.;B. J. Lee;K. S. Kang;J. H. Tai;J. J. Cho;M. H. Cho;T. Inoue;Y. S. Lee
  20. J. Bacteriol. v.181 MinDE-dependent pole-to-pole oscillation of division inhibitor MinC in Escherichia coli Raskin, D. M.;P. A. J. de Boer
  21. Arch. Biochem. Biophys. v.284 Molecular characterization of an anion pump; Purification of the ArsC protein Rosen, B. P.;U. Weigel;R. A. Monticello;B. P. F. Edwards
  22. Nucleic Acids Res. v.18 Identification of the metalloregulatory element of the plasmid-encoded arsenical resistance operon San Francisco, M. J. D.;C. L. Hope;J. B. Owolabi;L. S. Tisa;B. P. Rosen
  23. J. Biol. Chem. v.265 Molecular characterization of an anion pump Tisa, L.;B. P. Rosen
  24. Mol. Microbiol. v.5 The ArsR protein is a transacting regulatory protein Wu, J.;B. P. Rosen
  25. J. Biol. Chem. v.268 Metalloregulated expression of the ars speron Wu, J.;B. P. Rosen