Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Characterization of Quorum-Sensing Signaling Molecules Produced by Burkholderia cepacia G4

  • Park, Jun-Ho (Department of Microbiology, Changwon National University) ;
  • Hwang, In-Gyu (School of Agricultural Biotechnology, Seoul National University) ;
  • Kim, Jin-Wan (Department of Microbiology, Changwon National University) ;
  • Lee, Soo-O (Department of Microbiology, Changwon National University) ;
  • Conway, B. (Department of Microbiology, University of Iowa) ;
  • Peter Greenberg, E. (Department of Microbiology, University of Iowa) ;
  • Lee, Kyoung (Department of Microbiology, Changwon National University)
  • Published : 2001.10.01
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Abstract

In many Gram-negative bacteria, autoinducers, such as N-acyl-L-homoserine lactone(acyl-HSL) and its derivative molecules, mediate the cell-density-dependnet expression of certain operons. The current study identified the autoinducers produced by Burkholderia cepacia G4, a trichloroethylene-degrading lagoon isolate, using TLC bioassays with Agrobacterium tumefaciens NT1(pDCI141E33) and Chromobacterium violaceum CVO26, and a GC-MS analysis. The ${R_f}\;and\;{R_t}$ values and mass spectra were compared with those of synthetic compounds. Based on the analyses, it was confirmed that G4 produces N-hexanoyl (C6)-, N-octanoyl (C8)-, N-decanoyl (C10)-, N-dodecanoyl (C12)-HSL, and an unknown active species. The integration of the GC peak areas exhibited a ratio of C8-HSL:C10-HSL:C12-HSL at 3:17:1 with C6-HSL and C10-HSL production at trace and micromolar levels, respectively, in the culture supernatants. Nutants partially defective in producing acyl-HSLs were also partially defective in the biosynthesis of an antibiotic substance. These results indicate that the autoinducer-dependent gene regulation in G4 is dissimilar to the clinical B. cepacia strains isolated from patients with cystic fibrosis.

Keywords

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