New Expanded Bed Adsorption for Purification of Bovine Serum Albumin

  • Hu, Hong-Bo (College of Life Science and Biotechnology, Shanghai Jiaotong University) ;
  • Yao, Shan-Jing (Department of Chemical and Biochemical Engineering, Zhejiang University) ;
  • Zhu, Zi-Qiang (Department of Chemical and Biochemical Engineering, Zhejiang University) ;
  • Hur, Byung-Ki (Department of Biological Engineering, Inha University)
  • Published : 2001.10.01

Abstract

Based on the static and dynamic adsorption of Streamline DEAE, a modified tank-in-series model including particle size distribution was used to describe the adsorption performance of bovine serum albumin in an expanded bed. The calculated results indicated that the suggested model was able to simulate breakthrough curves under various conditions.

Keywords

References

  1. Enzyme Microb. Technol. v.23 Simple two-step procedure for purification of cloned small sialidase from unclarified E. coli feedstocks Chang, Y. K.;Y. H. Chen;C. H. Chien
  2. J. Chromatogr. v.297 Prediction of the performance of preparative affinity chromatography Chase, H. A.
  3. Trends Biotechnol. v.12 Purification of proteins by adsorption chromatography in expanded beds Chase, H. A.
  4. Biotechnol. Bioeng. v.67 Facilitated downstream processing of a histidine-tagged protein from unclarified E. coli homogenates using immobilized metal affinity expanded bed adsorption Clemmitt, R. H.;H. A. Chase
  5. Poster presented at 7th Europe Congress on Biotechnology Physical-chemical properties of streamline ion exchanger Hasson, K. A.
  6. Chem. Eng. Res. Des. v.67 Modelling the affinity adsorption of immunoglobulin G to protein A immobilised to agarose matrices Horstmann, B. J.;H. A. Chase
  7. Chinese J. Chem. Eng. v.8 Adsorption performance of protein in an expanded bed Hu, H. B.;S.J. Yao;D. Q. Lin;Z. Q. Zhu
  8. Biotechnol. Lett. v.22 Partial purification of nattokinase from Bacillus subtilis by expanded bed adsorption Hu, H. B.;S. J. Yao;L. H. Mei;Z. Q. Zhu;B. K. Hur
  9. Biotechnol. Bioeng. v.55 The influence of particle size distribution and operating conditions on the adsorption performance in fluidized bed Karau, A.;C. Benken;J. Thommes;M. R. Kula
  10. Ind. Eng. Chem. Res. v.37 Pore and surface diffusion and bulk-phase mass transfer in packed and fluidized beds Koh, J. H.;P. C. Wankat;N. H. L. Wang
  11. Chem. Eng. Sci. v.54 Modeling of the continuous-current expanded bed adsorber for the purification of proteins Owen, R. O.;H. A. Chase
  12. AIChE J. Modeling of solid-liquid fluidization in the Stokes flow regime using two-phase flow theory Thelen, T. V.;W. F. Ramirez
  13. Biotechnol. Bioeng. v.69 Experimental measurement of particle size distribution and voidage in an expanded bed adsorption system Willoughby, N. A.;R. Hjorth;N. J. Titchener-Hooker
  14. Biotechnol. Prog. v.15 Effect of resin characteristics on fluidized bed adsorption of proteins Wright, P. R.;F. J. Muzzio;B. J. Glasser
  15. J. Microbiol. Biotechnol. v.9 Direct purification of lysozyme from Hen egg white using high density mixed mode adsorbent Kim, W. K.;B. H. Chung