Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Production of Xylooligosaccharides with Thermostable Xylanases from the Streptomyces thermocyaneo-violaceus

내열성 방성균 Streptomyces thermocyaneoviloaceus 의 Xylanases를 이용한 자일로올리고당의 생산

  • 이오석 (경북대학교 농과대학 농화학과) ;
  • 최충식 (경북대학교 농과대학 농화학과) ;
  • 최준호 (경북대학교 농과대학 농화학과) ;
  • 주길재 (경북대학교 농과대학 농화학과) ;
  • 이인구 (경북대학교 농과대학 농화학과)
  • Published : 2001.12.01
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Abstract

Streptomyces themocyaneovio-laceus producing the thermostable xylanase was used for the production of xylooligosaccharides from xylan. The optimal conditions for the xylanase production were investigated in jar fermentor, which operated at 2 vvm aera-tion and 400 rpm agitation speed at $50^{\circ}C$ for 24 h. The optimal reaction condtion for the production of xylooli-gosaccharides with xylanases which were prepared by the percipitation with ammonium sulfate were obtained by the reaction at $60^{\circ}C$ for 12 h in the mixture composed of 10% birchwood xylan in 50 mM sodium phosphate buffer (pH 6.0)and 10 unit/ml of xylanase. In this optimal condition for the xylooligosaccharides production the mixture of xylooligosaccharides (58.8 g/I) which were composed of 20.1 g/I of xyobiose, 8.9 g/I of xylotriose 4.5 g/I of xylotetraose 16.2g/I of xylopentaose and 9.1 g/I xylohexaose and 5.0 g/I of xylose was produced from 100 g/I of birchwood xylan by the xylanases of S thermocyaneoviolaceus .

S. thermocyaneoviolaceus 가 생산하는 xylanases를 황산암모늄에 의한 염석 및 투석에 의해 조제한 효소를 이용하여 자일로오리고당을 생산하기 위한 최적 반응조건을 설정하였다. 자알로올리고당 생산을 위한 기질로는 oat spelt xyaln 보다 birchwood xylan을 사용하는 것이 더 좋았다. 반응온도의 영향을 조사한 결과 효소의 열 안정성과 자일로올리고당의 생선량 등을 고려할 때 $60^{\circ}C$에서 반응하는 것이 가장 좋았다 효소의 농도 pH의 영향및 반응시간의 영향을 조사한 결과 10 unit/ml xylanases를 첨가하여 pH 6.0에서 12 시간 반응시켰을 때 가장 많은 양의 자일로올리고당을 생산할 수 있었다. 이러한 최적 반응 조건에서 10% birchwood xylan 으로 부터 생산할 수 있는 자일로올리고당의 조성는 X2, X3, X4, X5 및 X6가 각각 20.1, 8.9 , 4.5, 16.2 및 9.1 g/I 이었으며 이때 생산되는 총 자일로올리고당 (X2~X6)은 58.8 g/I 이었다.

Keywords

References

  1. Trends Biotechnol. v.3 Microbial xylanolytic systems Biely, P.
  2. Purification and characterization of xylanase from Streptomyces thermocyaneoviolaceus Thesis for the master of agriculture Choi, J. H.
  3. Agri. Res. Bull. v.16 Production of xylanase by Streptomyces thermocyaneoviolaceus Choi, J. H.;D. H. Kwon;O. S. Lee;G. J. Joo;H. D. Park;I. K. Rhee
  4. Enzyme Microb. Technol. v.18 Repeated treatments with Aureobasidium pullulans hemicelluloses and alkali enhance biobleaching of sulphite pulps Christov, L. P.;B. A. Prior
  5. Adv. Carbohydr. Chem. Biochem. v.32 Hemicellulases: their occurrence, purification, properties, and mode of action Dekker, R. F. H.;G. N. Richard
  6. Biotechnol. Bioeng. v.27 Purification and characterization of endo-xylanases from Aspergillus niger. Two isozymes active on xylan backbones near branch point Frederick, M. M.;C. H. Kiang;J. R. Frederick;P. J. Reilly
  7. Appl. Environ. Microbiol. v.60 Characterization and sequence of a Thermomonospora fusca xylanase Irwin, D.;E. D. Jung;D. B. Wilson
  8. Korean Soc. Food Sic. Nutri. v.27 Effect of dietary xylooligosaccharide on indigestion and retarding effect of bile acid movement across a dialysis membrane Joo, G. J.;I. K. Rhee;S. H. Kim;S. J. Rhee
  9. Korean Soc. Food Sci. Nutri. v.27 Effects of dietary xylooligosaccharides on lipid levels of serum in rats fed high cholesterol diet Kim, S. H.;I. K. Rhee;G. J. Joo;H. P. Ha;S. J. Rhee
  10. Agric. Biol. Chem. v.55 Does-response of isomaltooligosaccharide for increasing fecal Bifidobacteria Kohomoto, T.;F. Fukui;H. Takaku;Y. Machida;T. Mitsuoka
  11. Acta Microbiol. Sinica v.21 Studies on the classification of thermophilic Actinomyces. I. Determination of thermophilic Streptomycetes(2) Lu, Y.;X. Yan
  12. Anal. Chem. v.31 Use of dinitrosalicylic acid reagent for determination of reducing sugar Miler, G. L.
  13. Functional Foods Special physiological functions of newly developed mono and oligosaccharides Oku, T.;I. Goldberg(ed.)
  14. Basic Life Sci. v.18 Xylanase: structure and function Reilly, P. J.
  15. FEMS Microbiol. Rev. v.104 Molecular biology of xylan degradation Thomson, J. A.
  16. Microbiol. Rev. v.52 Multiplicity of β-1,4-xylanase in microorganisms: functions and applications Wong, K. K. Y.;L. U. L. Tan;J. N. Saddler
  17. Hemicellulose and Hemicellulases Applications of hemicellulases in the food, feed, and pulp and paper industries Wong, K. K. Y.;J. N. Saddler;P.P. Coughlen;G.P. Hazlewood(ed.)
  18. New Food Industry v.35 Application of xylooligosaccharides for the processed food Yasuda, T.