한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제29권4호
  • /
  • Pages.195-200
  • /
  • 2001
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Phytate 분해 유산균의 분리 및 이용

Isolation and Application of Phytate-Hydrolysing Lactic Acid Bacteria.

  • 발행 : 2001.12.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구는 단위 가축 사료로 이용되는 곡류 등에 존재하는 항연인자인 Phytate 함유 환경하에서의 성장 및 phytate 분해력이 우수한 유산균을 분리하고 발효사료 제조 및 가축 생균제용 균주로의 이용성을 검토하기 위하여 실시하였다. 분리 유산균은 자돈 분변으로부터 얻은 유산균들중 phytate 함유 환경하에서의 성장 및 phytate 분해력이 가장 우수한 균으로 동정결과Lactobacillus paracasei subsp, paracasei로 동정되었다. 분리 규주는 내산성, 내담증섭이 높고, 사료원료를 배지성분으로 하여 순수배양한 결과 대두박 및 밀기울의 phytate-P 분해율이 각각 27.07및 12.18%로 나타났다. 또한 분리 균주를 비육돈 사료에 첨가하여 급여한 결과 중체량 개선효과는 나타나지 않았으나 사료효율 개선효과를 얻을 수 있었다.

Among lactic acid bacteria isolated from young animal feces, a bacterium having high phytate degradation ability, identified as L. paracasei subsp. paracasei, was selected. When inoculated into soybean meal, wheat bran and rice bran, the bacterium showed phosphate group of phytate (phytate-P) degrad- ability of 27.07% for soybean meal and 12.18% for wheat bran. However, degradation of phytate-P was not observed for rice bran L. paracasei subsp, paracasei had good acid and bile juice tolerance, having 9.70, 9.66 and 8.80 (log CFU/ml) for control, acid and bile juice treatment. Feed efficiency increased from 3.71 to 3.21 with addi- tion of the bacterium at 0.4% (w/w) level in swine fattener\`s diets.

키워드

참고문헌

  1. Nutr. Rev. v.42 Phosphorus bioavailability in poultry nutrition Anon
  2. Anal. Biochem. v.72 A rapid and sensitive method for quantification of microgram quantities of protein utilizing the principle of protein-dye binding Bradford, M. M.
  3. Cereal Chem. v.62 Solubility behaviours of the minerals, proteins, and phytic acid in rice bran with time, temperature and pH Champagne, E. T.;R. M. Rao;J. A. Liuzzo;J. W. Robinson;R. J. Gale;F. Miller
  4. J. Agric. Food Chem. v.23 Identification and properties of phytate in cereal grains and oilseed products de Boland, A.;G. B. Garmer;B. L. O'Dell
  5. Biometrics v.11 Multiple range and multiple F tests Duncan, D. B.
  6. Poultry Sci. v.57 Interrelationship of Lactobacillus and zinc bacitracin in the diets of turkey poults Francis, C.;D. M. Janky;A. S. Arafa;R. H. Harms
  7. J. Sci. Food Agric v.34 Sensitive method for the rapid determination of phytate in cereals and cereal products Haug, W.;H. J. Lantzsch
  8. Bergey's Manual of Systematic Bacteriology v.2 Genus Lactobacilus Kandler, O.;N. Weiss;Kandler, O.(ed.);N. Weiss(ed.)
  9. J. Food Sci. v.55 Effects of gemination and pure culture fermentation by yeasts and lactobacilli on phytic acid and polyphenol content of pearl millet Khetarpaul, N.;B. M. Chauhan
  10. J .Sci. Food Agri. v.55 Effect of natural fermentation on phytate and polyphenolic content and in-vitro digestibility of starch and protein of pearl millet(Pennisetum typhoideum) Khetarpaul, N.;B. M. Chauhan
  11. Biotechnol. Lett. v.14 Utilization of phytate by some yeasts Lambrechts, C.;H. Boze;G. Moulin;P. Galzy
  12. Kor. J. Appl. Microbiol. Biotechnol v.25 Viability in artificial gastric and bile juice and antimicrobial activity of some lactic acid bacteria isolated from Kimchi. Lee, S. H.;M. J. No
  13. J. Food Sci. v.48 Release of phosphorus from phytate by natural lactic acid fermentation Lopez, Y.;D. T. Gordon;M. L. Fields.
  14. J. Agric. Food Chem. v.30 Phytate: its chemistry, occurrence, food interactions nutritional significance, and methods of analysis Maga, J. A.
  15. Nutr. Rep. Intl. v.27 Effect of source and level of dietary yeast product on phytate phosphorus utilization by rats fed low phosphorus diets Moore, R. J.;T. L. Veum
  16. Poultry. Sci. v.73 Phytase activity, phosphorus and calcium retention, and performance of single comb white leghorn layers fed diets containing two levels of available phosphorus and supplemented with directfed microbials Nahashon, S. N.;H. S. Nakaue;L. W. Mirosh
  17. J. Agric. Food Chem. v.41 Reduction of phytate content in wheat bran and glandless cotton flour by Schwanniomyces castellii Segueilha, L.;G. Moulin;P. Galzy
  18. Biosci. Biotech. Biochem. v.56 Purification and characterization of phytase from Bacillus subtilis (natto) N-77 Shimizu, M.
  19. Lett. Appl. Microbiol. v.19 Ability of some strains of lactic acid bacteria to degrade phytic acid Shiral, K.;S. Revah-Molseev;M. Garcia-Garlbay;V. M. Marshall
  20. J. Appl. Bacteriol. v.58 Phytic acid changes in soybeans fermented by traditional inoculum and six strains of Rhizopus oligosporus Sutardi;K. A. Buckle
  21. Poultry Sci. v.52 Influence of the implantation of Lactobacillus acidophilus in chicks on the growth, feed conversion, malabsorption of fats syndrome and intestinal flora Tortuero, F.