한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제29권3호
  • /
  • Pages.134-141
  • /
  • 2001
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  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Keratinolytic protease 생산균, Pseudomonas sp. KP-364의 분리 및 배양

Isolation of Keratinolytic Protease Producing Microorganism and Its Cultivation Condition

  • 전동호 (건국대학교 공과대학 미생물공학과) ;
  • 권태종 (건국대학교 공과대학 미생물공학과)
  • 발행 : 2001.09.01
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초록

경기도 일대의 가금류 공장부근 토양로부터 단백질 분해활성이 높은 균주를 분리한 후 이것들 가운데 Keratinolytic protease 생산성이 우수한 균주는 KP-364를 선별하여 효소생산을 위한 최적 배양조건을 검토하였다. 선별된 KP-364 는 형태학적 생화학적 특성을 종합한 결과 Pseudomonas sp 로 동정되었으며 편의상 Pseudomonas sp. KP-364로 명명하였다. 효소생산을 위한 최적 배지조성은 Keratin 0.1% glucose 2.0% soybeam meal 0.5% NaNO3 0.5% KCI 0.2%이었고 초기 pH 6.5 배양온도 $37^{\circ}C$, 48시간진탕 배양할때 효소생산이 가장 우수하였으며 이때의 효소의 역가는 1,270 U/ml/hr이었다.

A bacterial strain KP-364 producing extracellular keratinolytic protease was isolated from the soil of the poultry fac-tory. It was identified as Pseudomonas sp. based on its morphological and physiological characteristics, The optimal culture conditions for the production of keratinolytic protease by Pseudomonas sp. KP-364 were investigated. The composition of optimal medium for the keratinolytic protease was 2.0% glucose, 0.5% soybean meal. 0.5% $NaNO_3$ and 0.2% KCI Optimal initial pH for production of Keratinolytic protease production were 6.5 and $37^{\circ}C$ respec- tively. The keratinolytic protease production reached a maximum of 1,270 U/ml/hr after 48 hours cultivation under the optimal culture conditions.

키워드

참고문헌

  1. Appl. Environ. Microbiol v.61 Characterizaion of a keratinolytic serine proteinase from Streptomyces pactum DSM 40530 Brigitte, B.;B. Galunsky;R. Muller
  2. Prog. Histochem. Cytochem. v.25 Cohesion amd desquamation of epidermal stratum corneum Brysk, M. M.;S. Rajaraman
  3. Ann. Rev. Cell Develop. Biol v.11 Keratins and the skin Elaine, F.
  4. Comp. Biochem. Physiol v.77 Puripication and some properties of an acidic protease from epimastigtes of Trupanosoma cruzi Esteban, B.;M. Berta
  5. Med. Microbiol. Immunol v.180 Quantification of keratinolytic activity from Dermatophilus congolensis Hanel, H.;J. Kalisch;M. Keil;W. C. Marsch;M. Buslau
  6. Med. Microbiol. Immunol. v.180 Quantification of keratinolytic activity from Dermatophilus congolensis Heinz, H.;J. Kalisch;M. Keil;W. C. Marsch;M. Buslau
  7. Williams and Wilkins Bergey's Manual of Determinative Bacteriology, 9th ed. John, G. H.;N. R. Krieg;P. H. A. Sneath
  8. Keratinolysis by poultry farm soil fungi. Mycopathol v.139 Kaul, I.;G. Sumbali
  9. J. Med. Veter. Mycol. v.25 Intracellular keratinase of Trichophyton gallinae Krystyna. W.;J. Lobarzewski;T. Wolski
  10. Yonsei Med. J. v.28 Isolation, purification and characterization of keratinolytic proteinase from Microsporum canis Lee, K. H.;K. K. Park;S. H. Park;J. B. Lee
  11. Acta. Derm. Venereol. v.71 Stratum corneum chymotryptic enzyme: a proteinase which may be generally present in the stratum corneum and with a possible involvement in desquamation Lundstrom, A;T. Egelrud
  12. Biochem. J. v.232 Purification and characterization of major extracellular proteinases from Trichophyton rubrum Masakazu, A.;R. Lindquist;K. Fukuyama;F. Apodaca;W. L. Epstein;J. H. McKerrow
  13. Dermatol. v.196 The in vitro and in vivo production of a 31.5-kd keratiolytic subtilase from Microsporum canis and the clinical status in naturally infected cats Mignon, B. R.;A. F. Nikkels;G. E. Pierard;B. J. Losson
  14. Mycoses. v.40 Extracellular enzyme activities of dermatophytes and yeast isolates on solid media Muhsin, T. M.;A. H. Aubaid;A. H. Al-Duboon
  15. J. Bacteriol v.117 Phosphate-mediated alteration of the Microsporum gypseum germination protease specificity for substrate: Enhanced keratinase activity Page, W. J.;J. J. Stock
  16. Biodegr. v.37 digestion of keratin by Pseudomonas sp.Inter.Biodeter Rosa, S. P.;F. Bicca;P. C. Santos;S. K. Ferrao;H. Dewes;C. C. Gpylarde;C. Termignoni;R. W. S. P. Thomas
  17. J. Invest. Dermatol. v.53 Hair digestion by a keratinase of Trichophyton mentagrophytes Ruey, J. Y.;S. R. Harmon;F. Blank;S. Nat;S. Techn
  18. Arch. Biochem. Biophysics. v.135 Amino acid composition and specificity of a keratinase of Trichophyton mentagrophytes Ruey, J. Y.;S. R. Harmon;P. E. Wachter;F. Blank
  19. J. Dermatol. v.14 a New keratinolytic proteinase from clinical isolates of Trichophyton mentagrophytes Ryoji, T.;I. J. Ko;K. Matsuda;H. Ogawa
  20. Br. J. Dermatol. v.134 The role of two endogenous proteases of the stratum corneum in degradation of desmoglein-1 and their reduced activity in the skin of ichthyotic patients Suzuki, Y.;J. Koyama;O. Moro;I. Horii;K. Kikuchi;M. Tanida;H. Tanida;H. Tagami
  21. Arch. Dermatol. Res. v.285 Detection and characterization of endogenous protease associated with desquamation of stratum comerum Suzuki, Y.;J. Nomura;J. Hori;J. Koyama;M. Takahashi;I. Horri
  22. Microbial ecology. The prokaryotes A: handbook on habitats, isolation, and identification of bacteria Mortimer P. S.
  23. Biosci. Biotech. Biochem v.56 Degration of Human Hair by a thermostable alkaline protease form alkaliphilic Bacillus sp. No. AH-101 Tideto, T.;S. Nakamura;R. Aono;K. Horikoshi
  24. Mycoses. v.38 Comparative studies on keratinase production of Trichophyton mentagrophytes strains of animal origin Ute, S.;K. H. Bohm
  25. Appl. Environ. Microbiol. v.56 Isolation, identification, and characterization of a feather-degrading bacterium Williams, C. M.;C. S. Richter;J. M. Mackenzie;J. C. H. Shih
  26. Appl. Environ. Microbiol. v.58 Purification and characterization of a keratinase from a feather-degrading Bacillus licheniformis strain Xiang, L.;C. G. Lee;E. S. Casale;J. C. H. Shih
  27. Appl. Environ. Microbiol. v.61 Nucleotide sequence and expressing of KerA,the gene encoding a keratinolytin protease of Bacillus Iicheniformis PWD-1 Xing, L.;D. W. Kelemen;E. S.miller;J. C. H.Shih
  28. Can. J. Microbiol. v.21 Degradation of feather keratin by culture filtrates of Streptomyces fradiae Young, R. A.;R. E. Smith
  29. J. Bacteriol. v.96 Isolation amd Purification of an extracellular keratinase of Trichophyton muntagrophytes Yu, R. J.;S. R. Harmon;F. Blank