Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Antimicrobial Activity of Culture Filtrates from Pseudomonas aeruginosa KLP-2 Isolated from Cooling Tower-Water against Legionella pneumophila

냉각탑수에서 분리한 Pseudomonas aeruginosa KLP-2 배양여액의 Legionella Pneumophila에 대한 항균활성

  • 박은희 (부산광역시 보건환경연구원 미생물과) ;
  • 차인호 (부산광역시 보건환경연구원 미생물과) ;
  • 이상준 (부산대학교 미생물학과)
  • Published : 2001.09.01
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Abstract

Pseudomonas aeruginosa KLP-2 possessing antimicro- bial activity against Legionella pneumophila was isolated from cooling tower-waters. The culture filtrates of P. aeruginosa KLP-2 showed antimicrobial activity against L. pneumophila Vibro cholerae non-Ol. Bacillus cereus, Bacillus subtilis and staphylococcus aureus. The culture filtrates of P. aeruginosa KLP-2 showed the highest anti-microbial activity against. L pneumophila among micoorganisms tested in this study. The optimal conditions of temperature, pH carbon source and nitogen source to obtain maximal antimicrobial activity from culture filtrates of P. aeruginosa KLP-2 were determined to be 35$^{\circ}C$ pH 7.0 % of glycerol and 0.6% of proteose peptone respec- tively. The antimicrobial activity of culture filtrates from P. seruginosa KLP-2 was highest against L. pneumophila when cultivated with shaking for 24 h and without shaking for 4 day at 35$^{\circ}C$.

냉각탑수 중의 Legionella 속 균을 저감시킬 수있는 방법개발의 일환으로 L. pneumophila에 강한 항균성물질을 생산하는P. seruginosa KLP-2 균주를 냉각탑수로부터 분리 동정하였다 P.aeruginosa KLP-2 균주를 냉각탑수로부터 분리 동정하였다. P. aeruginosa KLP-2 균주의 배양여액은 L. pneumophila의 Vibro cholerae non-Ol Bacillus cereus, Bacillus subtilis 및 Staphylococcus aureus에 대해서도 항균성을 나타내었다. P. seruginosa KLP-2 균주 배양여액의 항균활성을 증가시키기 위한 최적조건은 0.1%의 $K_2$$HPO_4$와 0.05%의 $MgSO_4$$7H_2$O로 조성되 무기염배지에 탄소원으로써 1%의 glycerol, 질소원으로써, 0.6%의 proteose peptone이 첨가된 pH 7.0의 배지 조건에서 가장 높은 항균 활성을 나타내었으며 $35^{\circ}C$에서 20시간 진탕배양(50 rpm)한 후 4일간 정치 배양하였을 때 최대의 항균활성을 나타내었다.

Keywords

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