A Study on Extender and Lower Temperature Storage for Fresh-extended Porcine Semen

돼지 액상정액을 위한 희석액 및 저온보존에 관한 연구

  • 김명철 (충남대학교 수의과대학) ;
  • 김용준 (전북대학교 수의과대학) ;
  • 조정곤 (전북대학교 수의과대학) ;
  • 이수진 (충남대학교 수의과대학) ;
  • 이재일 (충남대학교 수의과대학) ;
  • 김인철 (농촌진흥청 축산기술연구소) ;
  • 손동수 (농촌진흥청 축산기술연구소)
  • Published : 2001.12.01

Abstract

The aim of this study was to investigate the effect of sperm preservation according to the various kinds of commercially available semen extenders and to investigate the effect of sperm preservation according to the various temperature storages of fresh-extended porcine semen. To investigate the effect of sperm preservation according to the various kinds of commercially available semen extenders, porcine semens diluted in 3 semen extenders, Beltsville Thawing Solution(BTS), Androhep and Kiev, were cooled at $8^{\circ}C$ storage temperature with a controlled cooling rate of $2-4^{\circ}C$/h. Motility, progressive motility, normal sperm(%) and sperm morphology were assessed comparatively. In motility and progressive motility, Androhep extenders revealed better result than other extenders. In normal sperm(%) and sperm morphology, 3 semen extenders revealed similar results. To investigate the effect of sperm preservation according to the various temperature storages of fresh-extended porcine semen, porcine semens diluted in BTS extender, were cooled at 3 storage temperatures($8^{\circ}C$, $12^{\circ}C$ and $17^{\circ}C$) with a controlled cooling rate of $2-4^{\circ}C$/h. Motility, progressive motility, normal sperm(%) and sperm morphology were assessed comparatively. In motility and progressive motility, $8^{\circ}C$ treatment group revealed better result than $12^{\circ}C$ and $17^{\circ}C$ treatment groups. In normal sperm(%) and sperm morphology, 3 temperatures of treatment groups revealed similar results.

Keywords

References

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