Effect of Heat Shock on Culture Method and Essential Amino Acid Free Medium of IVM-Derived Bovine Embryo

체외성숙된 소 배에서 배양방법과 필수 아미노산 무첨가 배지에서의 온도충격의 영향

This study was carried out to evaluate the effect of culture methods on development of embryos with each developmental stage after heat shock in bovine oocytes. The results obtained were as fellows. 1. The culture method after heat shock on development of embryos was better drop-culture than co-culture. 2. The medium without amino acids were not effect of heat sock on development of embryos but it was in need of amino acid during formation of blastocyst.