Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
권호 표지

A Ser/Thr Specific Protein Kinase Activates the Mouse Rantes Gene after Lipolpolysaccharide STimulation

  • Kim, Youn-Uck (Division of Applied Biological Sciences, Sun Moon University) ;
  • Kim, Youn-Hwoan (Department of Microbial Engineering, Kon-Kuk University) ;
  • An, Duek -Jun (Division of Applied Biological Sciences, Sun Moon University) ;
  • Kwon, Hyuk-Chu (Division of Applied Biological Sciences, Sun Moon University)
  • Published : 2001.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

Macrophages stimulated by lipopolysaccharide(LPS) from gram negative bacteria undergo activation of a group of immediate early genes including Rantes. The mouse Rantes gene promoter region contains an LPS rsponsive element(LPE) We detected 3 specific bands termed B1, B2 and 3 formed by the interaction of the LPE and proteins found in LPS-stimulated RAW 367.7 cells. An additional band B4 was determined to be an Ap-1 binding protein. The B1 band appears within 1 hour of LPS nuclear extracts from LPS-stimulation, and this protein kinase enhances B1 and formation. The B1 band can be converted to band B2/B3 by adding specific heparin column fraction purified Ser/Thr specific protein phosphatases PP-1 and PP-2A can stimulate the same conversion to about the same extent. Thus, the formation of the LRE sequence binding complex appears to be regulated by Ser/Thr protein kinase and one or more Ser/Thr specific phosphatases. At least four proteins are involved in the trgulation of the LRE-dependent Rants experssion: two binding factors that bind directly to the target sequences. and two factors that control their binding. The future purification and characterization of these binding pro-teins will reveal in detail the mechanism of Rantes gene activation after LPS stimulation.

Keywords

References

  1. Infect. Immun. v.63 Induction of serine and threonine protein phosphorylation by Endotoxin-associated protein in murine resident peritoneal macrophage Abu-Lawi K.;B.M. Sultzer
  2. Adv. Immunol. v.54 Interleukin-6 in biology and medicine Akira S.;T. Taga;T. Kishimoto
  3. J. Immunol. v.155 Lipopolysaccharide stimulates the tyrosine phosphorylation of mitogen-activated protein kinases, in vascular endothelial cells in a soluble CD14-dependent manner, Role of protein tyrosine phosphorylation in lipopolysac-charide-induced stimulation of endothelial cells Arditi M.;J. Zhou;M. Torres;D.L. Durden;M. Stins;K.S. Kim
  4. J. Pharmacol. Exp. Ther. v.231 Activation of AP-1 and of a nuclear redox factor Asano T.;H. Hitaka
  5. Biochem. J. v.256 Inhibitory effect of a marine-sponge toxin, okadaic acid, on protein phosphatase Bialojan C.;A. Takai
  6. Trends Biochem. Sci. v.15 Okadaic acid, anew probe for the study of cellular regulation Cohen P.;C.F.B. Homes;Y. Tsukitani
  7. J. Immunol. v.152 Cloning, genomic organization, and chromosomal localization of the scya5 gene coding the murine chemokine Rantes Danoff T.M.;P.A. Lalley;Y.S. Chang;P.S. Heeger;E.G. Neilson
  8. Nucleic Acids Res. v.11 Accurate transcription initiation by RNA polymerase Ⅱ in a soluble extract from mammalian nuclei Dignam J.P.;R.M. Lebowitz;R.G. Roeder
  9. Immunol. Today v.18 Molecules and mechanisms operating in septic shock: lessons from knockout mice Gutierrez-Ramos J.C.;H. Bluethmann
  10. J. Exp. Med. v.179 Lipopolysaccharide (LPS)-binding protein accelerates the binding of LPS to CD14 Hailman E.;H.E. Lichenstein;H.S. Lichenstein;M.M. Wurfel;D.S. Miller;D.A. Johnson;M. Kelley;L.A. Busse;M.M. Zukowski;S.D. Wright
  11. J. Biol. Chem. v.260 IFN-gamma modelates PKC activity in murine peritoneal macrophage Hamilton T.A.;D.L. Becton;S.D. Somers;P.W. Gray;D.O. Adams
  12. Nature v.337 Effects of the tumour promoter okadaic acid on intracellular protein phosphorylation and metabolism Haystead T.A.;A.T.R. Sim;D. Carling;R.C. Honnor;Y. Tsukitani;P. Cohen;D.G. Hardie
  13. Kidney Int. v.41 Isolation and characterization of cDNA from renal tubular epithelium encoding murine Rantes Heeger P.;G. Wolf;C. Meyers;M.J. Sun;S.C. O'Farrel;A.M. Krensky;E.G. Neilson
  14. Biochem. v.23 Isoquinolinesulfonamides, novel and potent inhibitors of cyclic nucleotide dependent protein kinase and protein kinase C. Hidaka H.;M. Inagaki;S. Kawamoto;Y. Sasaki
  15. Cell v.80 Protein kinases and phosphatases: The yin and yang of protein phosphorylation and signaling Hunter T.
  16. Semin. Cell Biol. v.54 1001 protein kinase redux; towards 2000 Hunter T.
  17. J. Biol. Chem. v.259 Serotonin secretion from human platelets may be modifies by Ca²+ -activated, phospholipid-dependent myosin phosphorylation Inagaki M.;S. Kawamoto;H. Hidaka
  18. Biochem. Biophys. Res. Commun. v.159 Calyculin A and Okadaic acid: inhibitors of protein phosphatases activity Ishihara H.;B.L. Martin;D.L. Brautigan;H. Karaki;H. Ozaki;Y. Kato;N. Fusetani;S. Watabe;K. Hashimoto;D. Uemura;D.J. Hartshorne
  19. J. Exp. Med. v.176 Cytokine Rantes released by thrombin-stimulated platelets is a potent atraction for human eosinophils Kameyoshi Y.;A. Dorschner;A.I. Mallet;E. Christophers;J.M. Schroder
  20. Biochem. Biophys. Res. Communi. v.125 1-(5-Isoquinolinesulfonyl)-2-methylpiperazine(H-7) is a selective inhibitor of protein kinase C in rabbit platelets Kawamoto S.;H. Hidaka
  21. Immunopharmacology v.29 CD14 and other recognition molecules for lipopolysaccharide Kielian T.L.;F. Blecha
  22. Macrophage-mediated antibody-dependent cellular cytotoxity Koren H.S.
  23. J. Immunol. v.149 Rantes, a monocyte and T lymphocyte chemotatic cytokine releases histamine from human basophils Kuna P.;S.R. Reddigari;T.J. Schall;D. Rucinski;M.Y. Viksman;A.P. Kapkan
  24. Mol. Cell Biol. v.19 Essential role of interferon regulatory factor 3 in direct activation of RANTES chemokine transcription Lin R.;C. Heylbroeck;P. Genin;P.M. Pitha;J. Hiscott
  25. Trends Biochem. Sci. v.19 Inhiitors of protein kinases and phosphatases Mackintosh C.;R.W. Mackintosh
  26. Cell v.76 Subversion of immune system by pathogen Marrack P.;J. Kappler
  27. Trends. Cell Bio. v.4 Protein phosphatase 2A Mayer-Jaekel R.E.;B.A. Hemmings
  28. Adv. Immunol. v.28 Bacterial endotoxins and host immune responses Morrison D.C.;J.L. Ryan
  29. Annu. Rev. Biochem. v.61 Animal cell cycles and their control Norbury C.;P. Nurse
  30. Annu. Rev. Immunol. v.9 Properties of the novel proinflammatory supergene "intercrine" cytokine family Oppenheim J.J.;C.O.C. Zachariae;N. Mukaida;K. Matsushima
  31. Nucleic Acids Res. v.21 An EMSA-based method for determining the molecular weight of a protein-DNA complex Orchrd K.;G.E. May
  32. J. Immunol. v.156 Activation of multiple proline-directed kinases by bacterial lipopolysaccharide in murine macrophages Sanhera J.S.;S.L. Weinstein;M. Aluwalia;J. Girn;S.L. Pelech
  33. J. Immunol. v.141 A human T-cell-specific molecule is a member of a new gene family Schall T.J.;J. Jongstra;B.J. Bradley;J. Jorgensen;C. Clayberger;M. Davis;A.M. Krensky
  34. Nature v.347 Selective attraction of monocytes and T lymphocytes of the memory phenotype by cytokine Rantes Schall T.J.;K. Bacon;K.J. Toy;D.V. Goeddel
  35. Eur. J. Immunol. v.22 Molecular cloning and expression of the murine Rantes cytokine: strucutral and functional conservation between mouse and man Schall T.J.;N.J. Simpson;J.Y. Mak
  36. Mol. Cell. Biol. v.14 Definition of a lipopolysaccharide-resposive element in the 5'-flanking regions of MuRantes and crg-2 Shin H.S.;D. Beth-Ellen;L.S. Moon;W.N. Paul;N.F. Stephen;A.P. William
  37. Science v.260 Preferential migration of activated CD 4 and CD8 T cells in response to MIP-lalpha and MIP-1beta Taub D.D.;K. Conlon;A.R. Llyod;J.J. Oppenheim;D.J. Kelvin
  38. J. Immunol. v.151 Lipopolysaccharide-induced protein tyrosine phosphorylation in human macrophage is mediated by CD14 Weinstein S.L.;C.H. June;A.L. DeFranco
  39. J. Biol. Chem. v.259 The activation of protein kinase C by biologically active lipid A moieties of LPS Wightman P.;C.R.H. Raetz
  40. FASEB J. v.3 Macrophage infiammatory proteins 1 and 2: members of a novel superfamily of cytokines Wolpe S.D.;A. Cerami
  41. Science v.249 CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein Wright S.D.;R.A. Ramos;P.S. Tobis;R.J. Ulevitch;J.C. Mathison
  42. EMBO J. v.11 Identification and characterization of Ref-1, a nuclear protein that facilitates AP-1 DNA-binding activity Xanthoudakis S.;T. Curran
  43. EMBO J. v.11 Redox activation of Fos-Jun DNA binding activity is mediated by a DNA repair enzyme Xanthoudakis S.;G. Miao;F. Wang;E. Pan;Y.C.;Curran T.
  44. Mol. Cell. Biol. v.14 Activation of AP-1 and of a nuclear redox factor, Ref-1, in the response of HT29 colon cancer cells to hypoxia Yao K.S.;S. Xanthoudakis;T. Curran;P.J. O'Dwyer
  45. Biochem. Biophys. Res. Commun. v.166 A synthetic peptide substrae for selective assay for protein kinase C. Yasuda I.;A. Kishimoto;S.I. Tanaka;M. Tominaga;A. Sakurai;Y. Nishizuka