Effects of Aeration Rates on Production on Extracellular Polysaccharide, EPS-R, by Marine Bacterium Hahella chejuensis

  • Lee, Hyung-Sang (Microbiology Laboratory, Korea Ocean Research & Development Institute) ;
  • Park, Shin-Hye (Microbiology Laboratory, Korea Ocean Research & Development Institute) ;
  • Lee, Jong-Ho (Department of Biology, Sung Kyun University) ;
  • Lee, Hong-Kum (Microbiology Laboratory, Korea Ocean Research & Development Institute)
  • Published : 2001.09.01

Abstract

The production of an extracellular polysaccharide, EPS-R, from the marine bacterium Hahella chejuensis was investigated at various aeration rates in a batch culture. Higher aeration rate resulted in enhanced EPS production and increased the viscosity of the culture broth. At an aeration rate of 1.5 vvm, EPS-R (12.2 g/L) was obtained with a yield (Y$\_$p/s) of 0.6 from the STN medium after 72 h of cultivation. The H. chejuensis cells changed rod morphology to a short-rod form in the stationary growth phase.

Keywords

References

  1. Appl. Environ. Microbial. v.56 Construction of lactose utilizing Xanthomonas campestris and production of xanthan gum from whey Fu, J.F.;Y.H. Tseng
  2. Carbohydr. Res. v.198 Structural studied of the capsular polysaccharide from Streptococcus Pneumoniae type 7A Irene, B.M.;P.E. Jansson;B. Lindberg
  3. Carbohydr. Res. v.197 Structural characterization of the exocellular polysaccharides from Cyanospira capsulata Marra, M.
  4. Enzyme Microbiol. Technol. v.12 Roles of Mn²+ and Ca²+ on alginate biosynthesis by Pseudomonas aeruginosa Martins, L.O.;L.C. Brito;S.C. Isabel
  5. Appl. Environ. Microbiol. v.64 Role of Streptococcus thermophilus MR-1C capsular extracellular polysaccharide in cheese moisture retention Low, D.;J.A.; Ahlgren;D. Horne;D.J. McMahon;C.J. Oberg;J.G. Broadbent
  6. J. Gen. Appl. Microbiol. v.35 The conditions required for exopolymer production by a psychrotrophic bacterium Freeoni, G.D.
  7. J. Ferment. Bioeng. v.80 Production and optimization of certain growth parameters for an extracellular polysacchride from Azotobacter cinellandii MTCC 2460 isolated from a plant rhizosphere Vermani, M.V.;S.M. Kelkar;M.Y. Kamat
  8. Appl. Microbiol. Biotechnol. v.48 Influence of phosphate on rhamnose-containing extracellular polysacchride rheology and production by KlebsiellaⅠ-714 Farres, J.;G. Caminal;J. Lopez-Santin
  9. Carbohydr. Polym. v.34 Simaltaneous production of two different gel-forming extracellular polysaccharide by Alteromonas strain originating from deep sea hydrothermal vents Samain, E.;M. Milas;L. Bozzi;G. Dubreucq;M. Rinauda
  10. Appl. Environ. Microbiol. v.64 Enhancement of extracellular polysaccharide production by Lactobacillus delbruekii subsp. bugaricus NCFB 2772 with a simplified defined medium Grobben, G.J.;I. Chin-Joe;V.A. Kitzen;I.C. Boles;F. Boer;J. Sikkema;M.R. Smith;J.A.M. De Bont
  11. Appl. Environ. Microbiol. v.64 Optimization of extracellular polysaccharide production by Lactobacillus delbrueckii sunsp. bulgaricus RR growth in a semidefined medium Kimmel, S.A.;Roberts, R.F.;G.R. Ziegler
  12. J. Ferment. Bioeng. v.85 Succinoglycan production by Agrobacterium tumefaciens Stredansky, M.;E. Conti;C. Bertocchi;M. Matuloca;F. Zanett
  13. Appl. Microbiol. Biotechnol. v.50 Screening and characterization of Lactobavillus strains producing large amount of extracellular polysaccharides Van Geel-Schutten, G.H.;F. Flesch;B. ten Brink;M.R. Smith;L. Dijkhuizen
  14. Microbios. v.94 Effect of complex nitrogen sources upon gellan production by Sphingomonas paucimobilis ATCC 31461 West, T.P.;B. Strohfus
  15. Appl. Enviorn. Microbiol. v.65 Biosynthesis of novel exopolymers by Aureobasidium pullulans Lee, J.W.;W.G. Yeomans;A.L. Allen;F. Deng;R.A. Gross;D.L. Kaplan
  16. Nippon Suisan Gakkaishi v.58 Conditions for production of an extracellular polysaccharide by a Klebsiella sp. isolated from river water Kawaguchi, K.;K. Satomi;M. Yokoyama;H.
  17. Process Biochem. v.33 The influence of environmental conditions on polysaccharide formation by Ganoderma lucidum in submerged cultures Yang, F.-C.;C.-B. Liau
  18. Biotechnol. Prog. v.12 Xanthan gum fermentation by Xanthomonas campestris immobilized in a novel centrifugal fibrous-bed bioreactor Yang, S.-T.;Y.-M. Lo;D.B. Min
  19. Biotechnol. Bioeng. v.54 Production of a high viscosity polysacchride. methylan, in a novel bioreactor Oh, D.-K.;J.-H. Kim;T. Yoshida
  20. Int. J. Syst. Evol. Microbiol. v.51 Hahella chejuensis gen. nov., sp. nov., an extracellular-polysaccharide-producing bacterium Lee, H.K.;J. Chun;E.Y. Moon;S.-H. Ko;D.-S. Lee;H.S. Lee;K.S. Bae
  21. Biotechnol. Bioprocess Eng. v.5 Optimal conditions for the production of exopolysaccharide by marine microorganism Hahella chejuensis Ko, S.-H.;H.S. Lee;S.H. Park;H.K. Lee
  22. Methods for General and Molecular Bacteriology Chemical analysis Daniels, L.;R.S. Hanson;J.A. Phillips;F. Gerhardt(ed.);R.G.E. Murray(ed.);W.A. Wood(ed.)N.R. Krieg(ed.)
  23. Biotechnol. Lett. v.18 Improved extracellular polysacchride production in fed-batch fermentation of Aureobasidium pullulans, with increased impeller speed Moscovici, M.;C. Ionescu;C. Oniscu;O. Fotea;P. Protopopescu;L.D. Hanganu
  24. Appl. Microbiol. Biotechnol. v.28 Oxygen requiremnt in pullulan fermentation Rho, D.;A. Mulchandini;J.H.T. Luong;A. LeDuy
  25. Biotechnol. Lett. v.11 Effect of oxygen on the rate of β-1,3-glucan microbial exopolysaccharied production Lawford, H.;J. Rouseau
  26. J. Ind. Microbiol. v.9 Enhanced glucan formation of filamentous fungi by effective mixing, oxygen limitation and fed-batch processing Rau, U.;E. Gure;E. Olszewski;F. Wagner
  27. Biotechnol. Bioeng. v.34 The influence of agitation rate on xanthan production by Xanthomonas campestris Peter, H.U.;H. Herbert;P.G.M. Hesselink;H. Lunsdorf;A. Schumpe;W.D. Deckwer
  28. Appl. Environ. Microbiol. v.64 Vibrio cholerae O1 strain TSI-4 produces the exopolysacchride materials that determine colony morphology, stress resistance, and biofilm formation Wai, S.N.;Y. Mizunoe;A. Takada;S.-I. Kowabata;S.-I. Yoshida