Culture Parameters for Nonactin Production by Streptomyces viridochromogenes JM-4151

  • Lee, Sang-Han (Korea Research Institute of Bioscience & Biotechnology (KRIBB)) ;
  • Lee, Dong-Sun (Department of Microbiology, College of Natural Sciences, Kyungpook National University) ;
  • Lee, Jin-Man (Department of Microbiology, College of Natural Sciences, Kyungpook National University) ;
  • Kim, Tae-Ho (Department of Microbiology, College of Natural Sciences, Kyungpook National University) ;
  • Kim, Jong-Guk (Department of Microbiology, College of Natural Sciences, Kyungpook National University) ;
  • Han, Kab-Cho (Department of Life Science, Uiduk University) ;
  • Lee, Jin-Sik (Department of Life Science, Uiduk University) ;
  • Kwon, Gi-Seok (School of Bioresource Science, Andong National University)
  • 발행 : 2001.04.01

초록

Nonactin is the parent compound of a group of ionophore antibiotics, that known as the macrotetrolides. In previous report, in th course of screening superoxide radical-generating compounds from microbial sources, we first screened Streptomyces viridochromogenes JM-4151 that produces nonactin. It was proved that nonactin is superoxide radical-producing compound. In present study, we examined the optimal culture conditions of nonacin. Th optimal culture conditions for nonactin production were as follows: 1% soluble starch, 1% yeast extract, 0.2% ammonium nitrate, 0.06% magnesium sulfate, 0.2% calcium carbonate, initial pH 7.0 at 28$^{\circ}C$ for 96 h. The highest nonactin production was achieved in the production medium of initial pH7.0 at 28$^{\circ}C$ for 96h. The threshold level of dissolved oxygen was found to be above 33.2% at 28$^{\circ}C$ when 1% soluble starch was used as a carbon source. These results suggest that S. viridochromogenes JM-4151 might be a possible strain for industrial nonactin producer.

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