International Journal of Industrial Entomology and Biomaterials
- 제3권1호
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- Pages.19-23
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- 2001
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- 3022-7542(pISSN)
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- 2586-4785(eISSN)
Isolation and Characterization of Two Mosquitocidal Bacillus thuringien- sis Strains Belonging to subsp. kurstaki and subsp. aizawai
- Roh, Jong-Yul (School of Agricultural Biotechnology, Seoul National University) ;
- Li, Ming-Shun (School of Agricultural Biotechnology, Seoul National University) ;
- Chang, Jin-Hee (School of Agricultural Biotechnology, Seoul National University) ;
- Shim, Hee-Jin (School of Agricultural Biotechnology, Seoul National University) ;
- Jin, Byung-Rae (College of Natural Resources and Life Science, Dong-A University) ;
- Je, Yeon-Ho (School of Agricultural Biotechnology, Seoul National University)
- 발행 : 2001.09.01
초록
Two B. thuringiensis strains, which possess mosquitocidal activities, were isolated from Korean soil samples and named K-1205-1 and K-1381-1. Serological studies indicated that K-1205-1 and K-1381-1 belonged to B. thuringiensis subsp. kurstaki (H3a3b3c) and subsp. aizawai (H7), respectively. K-1205-1 produced typical bipyramidal parasporal inclusions, but K-1381-1 produced irregular bipyramidal shape. Total plasmid DNA patterns analysis shewed that K-1205-1 and K- 1381-1 were different from their reference strains, subsp. kurstaki and subsp. aizawai, respectively, in high molecules, whereas their crystal protein patterns showed no difference. The cry gene contents of K-1205-1 and K-1381-1 were identical with those of the reference strains. Mosquitocidal activities of crystal proteins produced by K-1205-1 and K-1381-1 were significantly high by about 40-50 folds at
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