Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Highly Branched Glucooligosaccharide and Mannitol Production by Mixed Cultrue Fermentation of Leuconostoc mesenteroides and Lipomyces starkeyi

  • Yoo, Sun-Kyun (Engineering Research Institute, Chonnam National University) ;
  • Kim, Do-Man (Engineering, Research Institute, Division of Chemical Engineering and the Research Institute for Catalysis, Chonnam National University) ;
  • Day, Donal F. (Audubon Sugar Institute, Louisiana Agricultural Station, Louisiana State University Agricultural Center)
  • Published : 2001.08.01
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Abstract

The influence of process conditions on highly branched glucooligosaccharides production by mixed culture of Leuconostoc mesenteroides ATCC 13146 and Lipomyces starkeyi ATCC 74054 was studied. We divided the batch culture fermentations into two groups according to inoculation method. One-point inoculation was performed by coinoculation of L. mesenteroides and L. starkeyi at the ration of 10 to 1, and two-point inoculation by L. mesenteroides inoculation first and L. starkeyi inoculation after L. mesenteroides grew to the end of the log phase of growth. Two-point inoculation improved the yield of oligosaccharide by 1.5 to 20 fold more than one-point inoculation. In this process, the highest yield of oligosaccharides (48% of theoretical yield) and productivity (0.85 g/l/h) were obtained with starch as an initial substrate for L. starkeyi growth. The estimated composition of the end product consisted of 31.5% oligosaccharides, 17.6% dextran, and 46.5% mannitol.

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References

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