Cloning of a Paenibacillus sp. Endo-${\circ}$-1,4-Glucanase Gene and Its Coexpression with the Endomyces fibuliger ${\circ}$-Glucosidase Gene in Saccharomyces cerevisiae

  • KIM, HYUNJIN (Division of Biological Sciences, College of Natural Sciences, Pusan National University) ;
  • JI-YOUNG YANG (Division of Food Science and Biotechnology, Pukyung National University) ;
  • HYEON-GYU LEE (Department of Food and Nutiriotion, Hanyang University) ;
  • JAEHO, CHA (Division of Biological Sciences, College of Natural Sciences, Pusan National University)
  • 발행 : 2001.08.01

초록

A gene, Egl, from Paenibacillus sp. KCTC 8848P encoding endo-${\circ}$-1,4-glucanase was cloned and expressed in Escherichia coli. It consisted of an open reading frame of 1,191 bp for a protein that consisted of 397 amino acids with a molecular weight of 44,539 Da. The deduced amino acid sequence of the endo-${\circ}$-1,4-glucanase gene had a 94% similarity to the endo-$\beta$-1,4-glucanase of Bacillus polymyxa. The Egl gene was also expressed in Saccharomyces cerevisiae secreting Endomyces fibuliger $\beta$-glucosidase (BGL1) under the control of the alcohol dehydrogenase (ADC1) gene promoter, S. cerevisiae transformant producing both endo-${\circ}$-1,4-glucanase and ${\circ}$-glucosidase grew on carboxymethyl cellulose as the sole carbon source.

키워드

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